| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF550 |
| Uniprot No | Q7Z398 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-413 aa |
| 活性数据 | MLVTFKDVAVTFTREEWRQLDLAQRTLYREVMLETCGLLVSLGHRVPKPELVHLLEHGQELWIVKRGLSHATCAGDRAQVHTREPTTYPPVLSERAFLRGSLTLESSTSSDSRLGRARDEEGLLEMQKGKVTPETDLHKETHLGKVSLEGEGLGTDDGLHSRALQEWLSADVLHECDSQQPGKDALIHAGTNPYKCKQCGKGFNRKWYLVRHQRVHTGMKPYECNACGKAFSQSSTLIRHYLIHTGEKPYKCLECGKAFKRRSYLMQHHPIHTGEKPYECSQCRKAFTHRSTFIRHNRTHTGEKPFECKECEKAFSNRAHLIQHYIIHTGEKPYDCMACGKAFRCSSELIQHQRIHTGEKPYECTQCGKAFHRSTYLIQHSVIHTGEMPYKCIECGKAFKRRSHLLQHQRVHT |
| 分子量 | 73.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于ZNF蛋白家族研究合成的示例参考文献(注：部分文献信息为假设性示例，建议核实真实数据库获取准确结果)：
1. **"Cloning and Functional Analysis of ZNF550 as a Novel Transcriptional Repressor in Human Cells"**
- 作者：Zhang, L., et al. (2018)
- 摘要：研究首次报道了重组人ZNF550蛋白的克隆及体外表达，发现其通过结合特定DNA序列抑制靶基因转录，可能与细胞周期调控相关。
2. **"ZNF550 Suppresses Tumor Metastasis by Regulating Epithelial-Mesenchymal Transition in Gastric Cancer"**
- 作者：Wang, Y., et al. (2020)
- 摘要：通过重组ZNF550蛋白功能实验，揭示其在胃癌中抑制EMT进程和转移的分子机制，提示其抑癌蛋白潜力。
3. **"Structural Characterization of Zinc Finger Protein 550 and Its Interaction with Chromatin Modifiers"**
- 作者：Lee, S., & Kim, H. (2021)
- 摘要：利用重组ZNF550蛋白进行结构解析，发现其锌指结构域与组蛋白去乙酰化酶(HDACs)互作，可能参与表观遗传调控。
4. **"Genome-wide Binding Profiling of ZNF550 Reveals Roles in Cellular Stress Response"**
- 作者：Chen, X., et al. (2019)
- 摘要：通过ChIP-seq分析重组ZNF550过表达细胞，鉴定其靶向氧化应激相关基因，提示其在DNA损伤应答中的作用。
**注意**：以上文献为模拟示例，实际研究中ZNF550可能研究较少。建议通过PubMed或Google Scholar以“ZNF550”“recombinant ZNF550”等关键词检索真实文献，或扩展至锌指蛋白家族(如ZNF268、ZNF384等)相关研究。
Zinc finger protein 550 (ZNF550) is a member of the zinc finger (ZNF) protein family, characterized by conserved cysteine-rich domains that facilitate sequence-specific DNA or RNA binding. As a Krüppel-associated box (KRAB)-containing ZNF protein, ZNF550 likely functions as a transcription regulator, mediating interactions between chromatin-modifying complexes and target gene promoters to influence epigenetic silencing or activation. The human ZNF550 gene is located on chromosome 19q13.43 and encodes a protein with an N-terminal KRAB domain, which often recruits co-repressors, and C-terminal tandem C2H2-type zinc finger motifs. While its precise biological roles remain understudied, ZNF550 may participate in cell differentiation, proliferation, or stress responses, as suggested by the regulatory roles of homologous KRAB-ZNF proteins in development and disease. Recombinant human ZNF550 protein is typically expressed in vitro (e.g., E. coli or mammalian systems) for functional studies, antibody production, or screening interactions with nucleic acids or proteins. Its structural features and tissue-specific expression patterns, detectable in organs like the placenta and testes, hint at context-dependent regulatory functions. Further research is needed to elucidate ZNF550’s molecular targets, physiological relevance, and potential involvement in pathological conditions such as cancer or neurological disorders.
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