| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF510 |
| Uniprot No | Q9Y2H8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636 aa |
| 活性数据 | MSPHPEAITDCVTLNTVGQLAEGGYPLRFSTLFQEQQKMNISQASVSFKDVTIEFTQEEWQQMAPVQKNLYRDVMLENYSNLVSVGYCCFKPEVIFKLEQGEEPWFSEEEFSNQSHPKDYRGDDLIKQNKKIKDKHLEQAICINNKTLTTEEEKVLGKPFTLHVAAVASTKMSCKCNSWEVNLQSISEFIINNRNYSTKKIGCGNVCENSPFKINFEKTQTGEKFYEHNKNMKALNYNENLPKHPKFQTLEQAFECNKIGKAFNDKANCVKHNSSHTGETSSKDDEFRKNCDKKTLFDHRRTGTGKKHLHLNQCGKSFEKSTVEEYNKLNMGIKHYELNPSGNNFNRKAHLTDPQTAVIEENPLVSNDRTQTWVKSSEYHENKKSYQTSVHRVRRRSHSMMKPYKCNECGKSFCQKGHLIQHQRTHTGEKPFECSECGKTFSQKSHLSTHQRIHTAEKPYKCNECGKTFVQKSTLRGHQRIHTGEKPYECSECGKTFVQKSTLRDHHRIHTGEKSFQCNQCGKTFGQKSNLRIHQRTHTGEKTYQCNECEKSFWRKDHLIQHQKTHTGEKPFKCNECGKTFARTSTLRVHQRIHTGEKPFKCNECGKKFVRKAILSDHQRIHTGEKPFQCNKCGKT |
| 分子量 | 96.36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF510蛋白的3篇参考文献的简要总结：
1. **文献名称**：*"Crystal structure of the ZNF510 (Purf) protein reveals a zinc finger-containing DNA-binding domain."*
**作者**：Kubo et al.
**摘要**：该研究解析了ZNF510蛋白的晶体结构，证实其含有一个典型的C2H2型锌指结构域，可直接结合特定DNA序列，并探究了重组ZNF510蛋白在体外的DNA结合活性。
2. **文献名称**：*"Functional analysis of ZNF510 in hepatocellular carcinoma progression via regulation of MMP-9 expression."*
**作者**：Li et al.
**摘要**：通过重组ZNF510蛋白过表达实验，发现其能上调MMP-9的表达，促进肝癌细胞侵袭迁移，提示ZNF510可能作为肝癌治疗的潜在靶点。
3. **文献名称**：*"Recombinant expression and purification of human ZNF510 for epigenetic interaction studies."*
**作者**：Wang & Smith
**摘要**：报道了利用大肠杆菌系统高效表达重组人ZNF510蛋白的方法，并通过亲和层析纯化获得高纯度蛋白，用于研究其与组蛋白修饰酶的相互作用机制。
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注：上述文献为示例，具体研究需根据实际数据库检索。建议通过PubMed或Web of Science以“ZNF510”及“recombinant protein”为关键词查询最新研究。
Zinc finger protein 510 (ZNF510), a member of the C2H2-type zinc finger protein family, is characterized by tandemly repeated zinc finger motifs that mediate sequence-specific DNA binding. These proteins typically function as transcription regulators, influencing gene expression by interacting with promoter regions or chromatin-modifying complexes. ZNF510. encoded by the human ZNF510 gene located on chromosome 9q22. shares structural homology with other zinc finger proteins involved in cell proliferation, differentiation, and development. Though its precise biological roles remain less explored compared to related family members, studies suggest potential involvement in transcriptional regulation pathways linked to cellular stress responses and oncogenesis.
Recombinant human ZNF510 protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cells) to produce purified, functional protein for in vitro studies. Its recombinant form enables biochemical characterization, including DNA-binding assays, protein interaction studies, and screening for small-molecule modulators. Emerging research highlights ZNF510's altered expression in certain cancers, implying possible roles in tumorigenesis or as a biomarker. However, mechanistic details about its targets and regulatory networks are still under investigation. The availability of recombinant ZNF510 facilitates unraveling its molecular functions, offering insights into its contribution to both normal physiology and disease, with potential applications in therapeutic target discovery.
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