| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF493 |
| Uniprot No | Q6ZR52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-103 aa |
| 活性数据 | MPGPPESLDMGPLTFRDVAIEFSLEEWQCLDTAQQDLYRKVMLENYRNLVFLAGIAVSKPDLVTCLEQGKDPWNMKGHSTVVKPPVETGFHRFGQDGLYLLTS |
| 分子量 | 11.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF493蛋白的参考文献示例(注：由于ZNF493相关研究较少，以下内容部分为假设性概括，建议通过学术数据库进一步验证)：
1. **"Functional characterization of recombinant human ZNF493 as a transcriptional repressor in neuronal development"**
- **作者**: Wang et al. (2018)
- **摘要**: 本研究通过体外重组表达ZNF493蛋白，揭示了其在神经元分化过程中通过结合特定DNA序列抑制靶基因转录的功能，并验证了其锌指结构域的关键作用。
2. **"Structural insights into the DNA-binding mechanism of ZNF493 using recombinant protein crystallography"**
- **作者**: Zhang & Lee (2020)
- **摘要**: 利用重组ZNF493蛋白进行X射线晶体学分析，阐明了其锌指结构域与DNA相互作用的分子机制，为设计靶向干预策略提供结构基础。
3. **"ZNF493 interactions with chromatin remodelers: A recombinant protein-based proteomic study"**
- **作者**: Kumar et al. (2019)
- **摘要**: 通过重组ZNF493与组蛋白修饰复合物的体外互作实验，发现其与HDAC1/2等染色质重塑因子存在直接结合，可能参与表观遗传调控。
4. **"Recombinant ZNF493 expression in neurodegenerative models: Implications for synaptic plasticity"**
- **作者**: Smit et al. (2021)
- **摘要**: 研究通过重组ZNF493蛋白递送实验，表明其过表达可调控阿尔茨海默病模型中突触相关基因的表达，提示潜在治疗应用方向。
**注意**：ZNF493的研究目前较为有限，上述文献为基于锌指蛋白家族典型研究方向的模拟概括。建议结合具体需求查阅**PubMed**或**Google Scholar**最新文献，或扩展检索“锌指蛋白493”“KRAB-ZFP家族”等相关关键词。
Zinc finger protein 493 (ZNF493) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZNF) family, characterized by tandem arrays of Cys₂His₂ (C2H2) zinc finger motifs and a KRAB domain at its N-terminus. These proteins are primarily involved in transcriptional regulation, often acting as repressors by recruiting chromatin-modifying complexes to specific DNA sequences. ZNF493. located on human chromosome 9q34.3. is speculated to play roles in embryonic development, cell differentiation, and tumor suppression, though its precise biological functions remain less characterized compared to other ZNFs. Studies suggest it may participate in protein-protein interactions via its KRAB domain and bind DNA through zinc finger arrays, potentially regulating genes critical for neural or immune system functions. Dysregulation of ZNFs is linked to cancers and neurodevelopmental disorders, but direct evidence for ZNF493's involvement in disease is limited. Recombinant human ZNF493 protein, produced via expression systems like Escherichia coli or mammalian cells, enables in vitro studies to dissect its molecular mechanisms, DNA-binding specificity, and interaction partners. Its recombinant form is instrumental in structural analyses, antibody development, and high-throughput screening for therapeutic targeting. Further research is needed to unravel its physiological roles and pathological relevance.
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