| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF498 |
| Uniprot No | Q6NSZ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544 aa |
| 活性数据 | MLKEHPEMAEAPQQQLGIPVVKLEKELPWGRGREDPSPETFRLRFRQFRYQEAAGPQEALRELQELCRRWLRPELHTKEQILELLVLEQFLTILPREFYAWIREHGPESGKALAAMVEDLTERALEAKAVPCHRQGEQEETALCRGAWEPGIQLGPVEVKPEWGMPPGEGVQGPDPGTEEQLSQDPGDETRAFQEQALPVLQAGPGLPAVNPRDQEMAAGFFTAGSQGLGPFKDMALAFPEEEWRHVTPAQIDCFGEYVEPQDCRVSPGGGSKEKEAKPPQEDLKGALVALTSERFGEASLQGPGLGRVCEQEPGGPAGSAPGLPPPQHGAIPLPDEVKTHSSFWKPFQCPECGKGFSRSSNLVRHQRTHEEKSYGCVECGKGFTLREYLMKHQRTHLGKRPYVCSECWKTFSQRHHLEVHQRSHTGEKPYKCGDCWKSFSRRQHLQVHRRTHTGEKPYTCECGKSFSRNANLAVHRRAHTGEKPYGCQVCGKRFSKGERLVRHQRIHTGEKPYHCPACGRSFNQRSILNRHQKTQHRQEPLVQ |
| 分子量 | 87.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的假设性参考文献示例(请注意文献为虚构，仅供格式参考)：
1. **"Functional Characterization of Recombinant Human ZNF498 in HEK293 Cells"**
- **作者**: Zhang, L. et al. (2018)
- **摘要**: 研究在大肠杆菌和HEK293细胞中重组表达人ZNF498蛋白，发现其通过调控转录因子AP-1活性影响致癌信号通路，证实其C端结构域在蛋白相互作用中的关键作用。
2. **"ZNF498 Knockdown Alters Neural Differentiation Pathways in vitro"**
- **作者**: Lee, S. & Kim, H. (2020)
- **摘要**: 利用CRISPR-Cas9技术构建ZNF498缺陷型细胞模型，发现重组ZNF498蛋白在神经分化中抑制Wnt/β-catenin通路，暗示其在神经发育中的潜在功能。
3. **"High-Throughput Screening Identifies ZNF498 as a DNA Damage Response Regulator"**
- **作者**: Chen, X. et al. (2021)
- **摘要**: 通过重组蛋白体外实验结合CRISPR筛选，揭示ZNF498通过与PARP1蛋白结合参与DNA损伤修复的分子机制。
4. **"Purification and Structural Analysis of Recombinant ZNF498 Zinc Finger Domains"**
- **作者**: Patel, R. & Gupta, A. (2019)
- **摘要**: 报道优化ZNF498锌指结构域的重组纯化方法，经X射线晶体学鉴定其特异性识别DNA基序的结构基础。
**备注**：上述文献为模拟内容，实际研究中请通过 **PubMed/Google Scholar** 等平台检索关键词(如：ZNF498 recombinant protein、ZNF498 function)获取真实文献。
Zinc finger protein 498 (ZNF498), also known as human C2H2-type zinc finger protein ZPR1. is a member of the zinc finger protein family characterized by conserved Cys₂His₂ (C2H2) motifs. These structural domains enable sequence-specific DNA or RNA binding, suggesting roles in transcriptional regulation, chromatin remodeling, or protein-protein interactions. While its precise biological functions remain under investigation, ZNF498 is implicated in cellular processes such as proliferation, differentiation, and stress response. Emerging evidence links dysregulation of ZNF498 to developmental disorders and cancers, though mechanistic details are sparse.
Recombinant human ZNF498 protein is typically produced using expression systems like *E. coli* or mammalian cell lines, followed by affinity chromatography purification. This engineered protein retains functional zinc finger domains, enabling *in vitro* studies to map DNA/RNA-binding sites, identify interaction partners (e.g., transcriptional co-regulators), and elucidate signaling pathways. Researchers utilize it to explore its role in gene regulatory networks, epigenetic modifications, and disease pathogenesis. Its recombinant form serves as a critical tool for structural analyses (e.g., X-ray crystallography) and high-throughput screening for potential therapeutic targets. Current research focuses on clarifying its tissue-specific functions and validating its proposed dual roles in tumor suppression and oncogenesis across different cellular contexts.
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