| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF492 |
| Uniprot No | Q9P255 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MLENYRNLVFVGIAASKPDLITCLEQGKEPWNVKRHEMVAEPPVVCSYFARDLWPKQGKKNYFQKVILRRYKKCGCENLQLRKYCKSMDECKVHKECYNGLNQCLTTTQNKIFQCDKYVKVFHKFSNSNRHTIRHTGKKSFKCKECEKSFCMLSHLAQHKRIHSGEKPYKCKECGKAYNETSNLSTHKRIHTGKKPYKCEECGKAFNRLSHLTTHKIIHTGKKPYKCEECGKAFNQSANLTTHKRIHTGEKPYKCEECGRAFSQSSTLTAHKIIHAGEKPYKCEECGKAFSQSSTLTTHKIIHTGEKFYKCEECGKAFSQLSHLTTHKRIHSGEKPYKCEECGKAFKQSSTLTTHKRIHAGEKFYKCEVCSKAFSRFSHLTTHKRIHTGEKPYKCEECGKAFNLSSQLTTHKIIHTGEKPYKCEECGKAFNQSSTLSKHKVIHTGEKPYKYEECGKAFNQSSHLTTHKMIHTGEKPYKCEECGKAFNNSSILNRHKMIHTGEKLYKPESCNNACDNIAKISKYKRNCAGEK |
| 分子量 | 84.81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇模拟的关于重组人ZNF492蛋白的参考文献(注：实际文献可能有限，建议通过PubMed或Google Scholar验证)：
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1. **文献名称**: *Cloning and Expression of Recombinant Human ZNF492 in E. coli*
**作者**: Smith A, et al.
**摘要**: 该研究成功构建了ZNF492的重组表达载体，利用大肠杆菌系统高效表达并纯化出具有活性的重组蛋白，为后续功能研究提供工具。
2. **文献名称**: *DNA-Binding Specificity of ZNF492 Revealed by in vitro Assays*
**作者**: Zhang L, et al.
**摘要**: 通过电泳迁移实验(EMSA)和染色质免疫沉淀(ChIP)，证明重组ZNF492蛋白优先结合富含GC碱基的启动子区域，提示其可能参与基因转录调控。
3. **文献名称**: *ZNF492 Overexpression Promotes Tumor Cell Proliferation in Breast Cancer*
**作者**: Kim Y, et al.
**摘要**: 研究利用重组ZNF492蛋白处理乳腺癌细胞，发现其通过激活Wnt/β-catenin信号通路显著促进细胞增殖，提示其作为潜在致癌因子。
4. **文献名称**: *Structural Insights into ZNF492’s Zinc Finger Domains*
**作者**: Patel R, et al.
**摘要**: 采用X射线晶体学解析了重组ZNF492蛋白的锌指结构域，揭示了其与DNA结合的关键氨基酸残基，为设计靶向抑制剂奠定基础。
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**建议**：若需真实文献，可尝试以下关键词搜索：
`"ZNF492" + "recombinant"`、`"Zinc finger protein 492" + "expression"`、`"ZNF492 function"`，并筛选近年研究。部分结果可能集中于锌指蛋白家族共性研究，而非ZNF492特异性分析。
**Background of Recombinant Human ZNF492 Protein**
Zinc finger protein 492 (ZNF492) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, which is characterized by tandem Cys₂His₂ zinc finger motifs and a KRAB domain. These proteins predominantly function as transcription regulators, interacting with DNA or other proteins to modulate gene expression. ZNF492. located on chromosome 19q13.43. is encoded by a gene spanning approximately 30 kb and contains eight exons. Its structure includes a KRAB domain at the N-terminus, which often mediates transcriptional repression by recruiting co-repressor complexes, and multiple zinc finger motifs at the C-terminus for DNA binding.
Though less studied compared to other zinc finger proteins, ZNF492 is implicated in various biological processes, including cell differentiation, apoptosis, and development. Expression analyses reveal its presence in tissues such as the testis, ovary, and brain, suggesting potential roles in reproductive and neural systems. Recent studies highlight its possible involvement in cancer, with altered expression observed in tumors, though mechanistic insights remain limited.
Recombinant human ZNF492 protein, typically produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells), enables functional studies, such as DNA-protein interaction assays, cellular localization analyses, and screening for binding partners. Its recombinant form is crucial for elucidating molecular pathways and therapeutic targets, particularly in diseases linked to transcriptional dysregulation. Further research is needed to fully unravel its physiological and pathological significance.
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