| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF485 |
| Uniprot No | Q8NCK3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402 aa |
| 活性数据 | MLENYGNLVSVGLLSSKPKLITQLEQGAEPWTEVREAPSGTHAVEDYWFETKMSALKQSTSEASVLGERTKSVMMEKGLDWEGRSSTEKNYKCKECGKVFKYNSSFISHQRNHTSEKPHKCKECGIAFMNSSSLLNHHKVHAGKQPYRCIECGKFLKKHSTFINHQRIHSREKPHKCIECGKTFRKNSILLSHQRIHTGQKPYKCNDCGKAFAQNAALTRHERIHSGEKPFKCNKCGRAFRDNSTVLEHQKIHTGEKPYQCNECGKAFRKSSTLISHQRMHTGEKPYHCSKCGKSFRYSSSFAGHQKTHSGNKPYQCRDCGKAFTKSSTLTGHQRIHTGEKPYHCKKCGKAFRHSSGLVEHQRLHTGEKPYKCNECGKAFPRSSALKQHKKIHNKERAMKCS |
| 分子量 | 72.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF485蛋白的3篇模拟文献示例，基于该蛋白可能的研究方向及常见研究框架整理而成。**请注意，这些文献为假设性示例，实际文献需通过学术数据库验证**：
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1. **文献名称**：*"Cloning and Functional Characterization of Human ZNF485 as a Transcriptional Repressor"*
**作者**：Chen, L., et al. (2017)
**摘要**：本研究成功克隆并在哺乳动物细胞中表达了重组人ZNF485蛋白，证实其通过锌指结构域结合DNA特定序列，抑制靶基因转录，可能在发育调控中起关键作用。
2. **文献名称**：*"ZNF485 Suppresses Hepatocellular Carcinoma Metastasis via Modulating Wnt/β-Catenin Signaling"*
**作者**：Wang, Y., et al. (2019)
**摘要**：通过体外重组表达ZNF485.研究发现其能下调Wnt通路活性，抑制肝癌细胞迁移和侵袭，提示其作为肿瘤抑制因子的潜在治疗价值。
3. **文献名称**：*"Structural Analysis of ZNF485 Zinc Finger Domains Reveals DNA-Binding Specificity"*
**作者**：Kim, S., et al. (2020)
**摘要**：采用X射线晶体学解析了ZNF485的锌指结构域，揭示了其与DNA结合的分子机制，为设计靶向该蛋白的基因编辑工具提供了结构基础。
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**建议检索策略**：
- 在PubMed或Web of Science中使用关键词：**ZNF485 recombinant protein**, **ZNF485 AND cancer**, **ZNF485 structure/function**。
- 结合领域内知名期刊(如*Nature Genetics*, *Oncogene*)或专注转录因子的杂志进行筛选。
Zinc finger protein 485 (ZNF485) is a member of the zinc finger protein family, characterized by conserved Cys₂/His₂ zinc finger domains that enable sequence-specific DNA or RNA binding. As a transcription factor, ZNF485 is hypothesized to regulate gene expression by interacting with promoter regions or chromatin-modifying complexes, though its precise molecular mechanisms remain under investigation. The human ZNF485 gene is located on chromosome 10q11.21 and encodes a protein with multiple zinc finger motifs, suggesting roles in transcriptional regulation, cell differentiation, and immune responses.
Recombinant human ZNF485 protein is engineered using heterologous expression systems (e.g., E. coli, HEK293. or insect cells) for functional studies. Its production enables researchers to explore DNA-binding specificity, protein-protein interactions, and regulatory pathways in vitro. Emerging evidence links ZNF485 dysregulation to diseases; for example, altered expression has been observed in certain cancers and autoimmune disorders, implying potential roles in tumor suppression or immune modulation. However, its physiological targets and signaling networks are not fully elucidated.
Current research leverages recombinant ZNF485 to decipher its structure-function relationships, often combined with CRISPR/Cas9-mediated knockout models or RNAi-based silencing. Challenges include optimizing protein solubility due to its repetitive zinc finger domains. Understanding ZNF485's role may contribute to insights into transcriptional networks underlying development, oncogenesis, and immune regulation, highlighting its relevance in both basic and translational biomedical research.
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