| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF486 |
| Uniprot No | Q96H40 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216 aa |
| 活性数据 | MESLQFRDVAVEFSLEEWHCLDTAQQNLYRDVMLENYRHLVFLGIIVSKPDLITCLEQGIKPLTMKRHEMIAKPPVVCSHFAQDLWPEQSIKDSYQKVILRKFEKCGHGNLHFKKGCESVDECKLHKRGYNGLNQCLTTTQSKIFQCGKYVKVFHQFSNSKRHKRRHTEKKPLKYIEGDKAFNQSSTHTTHKKIDTGEKPYKCEECGKAFNRSSCL |
| 分子量 | 51.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF486蛋白的3-4条参考文献的示例(注：以下内容为虚构，仅供示例参考，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Structural and Functional Analysis of Recombinant Human ZNF486 Protein*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人ZNF486蛋白，通过X射线晶体学解析了其锌指结构域的3D结构。实验表明，ZNF486通过特异性结合DNA调控序列，参与胚胎干细胞分化相关基因的转录抑制。
2. **文献名称**: *ZNF486 Recombinant Protein Suppresses Tumor Growth via Epigenetic Regulation*
**作者**: Chen L, et al.
**摘要**: 作者利用哺乳动物细胞系统制备了高活性的重组ZNF486蛋白，发现其通过招募组蛋白去乙酰化酶(HDAC)复合物，抑制肝癌细胞中致癌基因的表达，在体外和小鼠模型中均表现出显著的抗肿瘤活性。
3. **文献名称**: *Expression and Role of ZNF486 in Neural Development: Insights from Recombinant Protein Studies*
**作者**: Zhang Y, et al.
**摘要**: 通过昆虫细胞表达系统获得重组ZNF486蛋白，研究其在小鼠大脑皮层神经元中的功能。结果提示ZNF486通过调控神经突触相关基因的甲基化，影响神经前体细胞的迁移与分化。
4. **文献名称**: *Zinc Finger Proteins as Therapeutic Targets: A Review on ZNF486 Applications*
**作者**: Johnson R, et al.
**摘要**: 综述了ZNF家族蛋白在疾病中的作用，重点讨论了重组ZNF486蛋白在基因编辑工具开发(如CRISPR-ZF嵌合体)和再生医学中的潜在应用，强调其结构可塑性优势。
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**注意**：以上文献信息均为假设性示例，实际文献需通过**PubMed、Web of Science或Google Scholar**等平台以关键词“ZNF486”、“recombinant ZNF486 protein”检索确认。
Zinc finger protein 486 (ZNF486) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which constitutes one of the largest groups of transcription regulators in humans. These proteins are characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, along with functional domains like KRAB, which often recruit epigenetic modifiers to repress gene expression. ZNF486 is encoded by the *ZNF486* gene located on chromosome 19q13.43 and is postulated to play roles in transcriptional regulation, chromatin remodeling, and cellular differentiation. Though less studied compared to other zinc finger proteins, ZNF486 is hypothesized to regulate genes involved in development, immune response, and oncogenesis through its DNA-binding and protein-interaction capabilities. Recombinant ZNF486 protein, typically produced in bacterial or mammalian expression systems, enables in vitro studies to dissect its molecular functions, such as DNA-protein interactions, subcellular localization, and partnerships with co-repressors like TRIM28. Dysregulation of ZNF486 has been tentatively linked to diseases, including cancers, though mechanistic insights remain limited. Current research focuses on elucidating its target genes, signaling pathways, and potential as a diagnostic or therapeutic biomarker. Recombinant variants may also facilitate high-throughput screening for small molecules modulating its activity. Further studies are needed to clarify its physiological and pathological significance.
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