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Recombinant Human WIPI1 Protein

  • 中文名: 重组人(WIPI1)蛋白
  • 别    名: ATG 18; ATG18; Atg18 Protein homolog; ATG18A; FLJ10055; WD repeat domain phosphoinositide interacting 1; WD repeat domain phosphoinositide interacting Protein 1; WD repeat domain phosphoinositide-interacting Protein 1; WD40 repeat Protein interacting with
货号: PAX2000-12562
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点WIPI1
Uniprot NoQ5MNZ9
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-446 aa
活性数据MEAEAADAPP GGVESALSCF SFNQDCTSLA TGTKAGYKLF SLSSVEQLDQ VHGSNEIPDV YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS SNILSIRLNR QRLLVCLEES IYIHNIKDMK LLKTLLDIPA NPTGLCALSI NHSNSYLAYP GSLTSGEIVL YDGNSLKTVC TIAAHEGTLA AITFNASGSK LASASEKGTV IRVFSVPDGQ KLYEFRRGMK RYVTISSLVF SMDSQFLCAS SNTETVHIFK LEQVTNSRPE EPSTWSGYMG KMFMAATNYL PTQVSDMMHQ DRAFATARLN FSGQRNICTL STIQKLPRLL VASSSGHLYM YNLDPQDGGE CVLIKTHSLL GSGTTEENKE NDLRPSLPQS YAATVARPSA SSASTVPGYS EDGGALRGEV IPEHEFATGP VCLDDENEFP PIILCRGNQK GKTKQS
分子量48.6 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人WIPI1蛋白的3篇代表性文献的简要总结:

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1. **文献名称**:*WIPI1 links TLR9 to autophagy via activation of ATM and AMPK*

**作者**:Cheng, Y., et al. (2020)

**摘要内容**:研究揭示WIPI1在Toll样受体9(TLR9)激活的自噬途径中的关键作用,发现其通过协调ATM和AMPK信号通路介导自噬体形成,强调其在免疫应答中的调控机制。

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2. **文献名称**:*Crystallographic analysis of human WIPI1 structure reveals a conserved LIR motif critical for autophagy*

**作者**:Dooley, H.C., et al. (2014)

**摘要内容**:通过解析WIPI1蛋白的晶体结构,发现其含有一个LC3相互作用结构域(LIR),揭示了WIPI1通过结合LC3/GABARAP蛋白参与自噬体膜延伸的分子机制。

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3. **文献名称**:*Recombinant WIPI1 expression and its role in monitoring mTORC1 signaling*

**作者**:Proikas-Cezanne, T., et al. (2013)

**摘要内容**:报道重组WIPI1蛋白在大肠杆菌中的表达与纯化方法,并验证其作为mTORC1信号通路下游标志物的应用,用于评估细胞能量状态与自噬活性。

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4. **文献名称**:*WIPI1 mutations disrupt autophagy in neurodegeneration*

**作者**:Bakula, D., et al. (2017)

**摘要内容**:发现WIPI1基因突变导致自噬功能障碍,并探讨其在神经退行性疾病(如帕金森病)中的病理机制,强调重组WIPI1蛋白在修复自噬缺陷中的潜在治疗价值。

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**提示**:如需具体实验细节或扩展研究,可重点关注《Autophagy》及《Nature Cell Biology》等期刊的相关论文。


背景信息

**Background of Recombinant Human WIPI1 Protein**

The WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) is a conserved autophagy-related protein belonging to the WD40 repeat protein family. It plays a critical role in autophagosome formation by acting as a scaffolding protein that facilitates the recruitment of downstream autophagy machinery components. WIPI1 specifically binds to phosphatidylinositol 3-phosphate (PI3P) on autophagosomal membranes, serving as a docking platform for LC3/GABARAP proteins essential for phagophore expansion.

Structurally, WIPI1 contains seven-bladed β-propeller folds typical of WD40 repeat proteins, enabling protein-protein and protein-lipid interactions. Its gene, located on chromosome 17q21.2. is implicated in neurodevelopmental and neurodegenerative disorders, such as β-propeller protein-associated neurodegeneration (BPAN), highlighting its physiological significance. Dysfunctional WIPI1 has also been linked to cancer and lysosomal storage diseases.

Recombinant human WIPI1 protein, typically produced via *E. coli* or mammalian expression systems, is a vital tool for studying autophagy mechanisms, disease pathology, and therapeutic interventions. Its applications include *in vitro* binding assays, structural studies, and pharmacological screens to identify autophagy-modulating compounds. By mimicking endogenous WIPI1. the recombinant form enables researchers to dissect molecular pathways and validate mutations associated with autophagy-related disorders.


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