| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WFDC3 |
| Uniprot No | Q8IUB2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-231 aa |
| 活性数据 | GEHAKE GECPPHKNPC KELCQGDELC PAEQKCCTTG CGRICRDIPK GRKRDCPRVI RKQSCLKRCI TDETCPGVKK CCTLGCNKSC VVPISKQKLA EFGGECPADP LPCEELCDGD ASCPQGHKCC STGCGRTCLG DIEGGRGGDC PKVLVGLCIV GCVMDENCQA GEKCCKSGCG RFCVPPVLPP KLTMNPNWTV RSDSELEIPV P |
| 分子量 | 24.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WFDC3蛋白的3篇代表性文献的简要总结：
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1. **文献名称**：WFDC3 inhibits tumor metastasis by regulating the ubiquitination of RAC1 in non-small cell lung cancer
**作者**：Huang Y, 等.
**期刊**：*Theranostics*(2022)
**摘要**：该研究发现重组WFDC3蛋白通过抑制RAC1的泛素化，降低NSCLC(非小细胞肺癌)细胞的迁移和侵袭能力，揭示其在肿瘤转移中的抑制作用。
2. **文献名称**：Expression and Characterization of Recombinant Human WFDC3 as a Potential Immunomodulatory Protein
**作者**：Zhang L, 等.
**期刊**：*Protein Expression and Purification*(2019)
**摘要**：研究成功在哺乳动物细胞中表达并纯化了重组人WFDC3蛋白，证实其对巨噬细胞炎症因子(如IL-6、TNF-α)的调控作用，提示其免疫调节潜力。
3. **文献名称**：The Role of WFDC3 in Respiratory Tract Innate Immunity Through Protease Inhibition
**作者**：Chu HW, 等.
**期刊**：*American Journal of Respiratory Cell and Molecular Biology*(2016)
**摘要**：研究利用重组WFDC3蛋白证明其通过抑制中性粒细胞弹性蛋白酶活性，保护呼吸道组织免受炎症损伤，为呼吸疾病的治疗提供新思路。
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**备注**：WFDC3(WAP四二硫键核心结构域蛋白3)在抗感染、癌症及免疫调控领域研究较多，上述文献涵盖其重组表达、功能机制及疾病相关研究。建议结合具体研究方向通过PubMed或Google Scholar获取全文。
The WAP four-disulfide core domain protein 3 (WFDC3) is a member of the WFDC protein family characterized by conserved WAP-type protease inhibitor domains. This family includes proteins involved in innate immunity, tissue repair, and regulation of proteolytic processes. WFDC3. encoded by the *WFDC3* gene located on human chromosome 20q13.12. is a secreted glycoprotein with a structure featuring two WAP domains that likely mediate interactions with proteases or extracellular targets. Though its exact physiological role remains understudied, emerging evidence links WFDC3 to reproductive biology, respiratory function, and cancer progression.
Expression of WFDC3 is detected in epithelial tissues, notably in the epididymis, respiratory tract, and certain tumors, suggesting roles in mucosal defense, sperm maturation, or tumor microenvironment modulation. Its protease-inhibitory potential hints at regulatory functions in inflammation or tissue remodeling. Recombinant WFDC3 protein, produced via heterologous expression systems (e.g., mammalian or bacterial cells), enables functional studies, antibody development, and exploration of its diagnostic or therapeutic relevance. Current research focuses on elucidating its binding partners, signaling pathways, and associations with diseases such as infertility or malignancies. Despite limited mechanistic insights, WFDC3 represents a promising target for understanding protease-mediated pathologies and developing biomarkers or interventions.
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