| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WHAMM |
| Uniprot No | Q8TF30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-809 aa |
| 活性数据 | MEDEQPDSLEGWVPVREGLFAEPERHRLRFLVAWNGAEGKFAVTCHDRTAQQRRLREGARLGPEPEPKPEAAVSPSSWAGLLSAAGLRGAHRQLAALWPPLERCFPRLPPELDVGGGGAWGLGLGLWALLWPTRAGPGEAALQELCGQLERYLGAAADGCGGATVRDALFPAEGGAADCESPREFRERALRARWVEADARLRQVIQGHGKANTMVALMNVYQEEDEAYQELVTVATMFFQYLLQPFRAMREVATLCKLDILKSLDEDDLGPRRVVALEKEAEEWTRRAEEAVVSIQDITVNYFKETVKALAGMQKEMEQDAKRFGQAAWATAIPRLEKLQLMLARETLQLMRAKELCLNHKRAEIQGKMEDLPEQEKNTNVVDELEIQFYEIQLELYEVKFEILKNEEILLTTQLDSLKRLIKEKQDEVVYYDPCENPEELKVIDCVVGLQDDKNLEVKELRRQCQQLESKRGRICAKRASLRSRKDQCKENHRFRLQQAEESIRYSRQHHSIQMKRDKIKEEEQKKKEWINQERQKTLQRLRSFKDKRLAQSVRNTSGSEPVAPNLPSDLSQQMCLPASHAVSVIHPSSRKTRGVPLSEAGNVKSPKCQNCHGNIPVQVFVPVGDQTHSKSSEELSLPPPPPPPPPPPPPPPPPPPPLRALSSSSQAATHQNLGFRAPVKDDQPRPLVCESPAERPRDSLESFSCPGSMDEVLASLRHGRAPLRKVEVPAVRPPHASINEHILAAIRQGVKLKKVHPDLGPNPSSKPTSNRRTSDLERSIKAALQRIKRVSADSEEDSDEQDPGQWDG |
| 分子量 | 89 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WHAMM蛋白的3篇关键文献及其摘要：
1. **文献名称**：WHAMM directs actin assembly during membrane remodeling
**作者**：Campellone K.G., et al.
**摘要**：该研究首次报道了WHAMM蛋白通过激活Arp2/3复合体，驱动肌动蛋白(actin)的成核和分支，调控膜重塑过程(如高尔基体囊泡形成)，揭示了其在细胞内膜运输中的作用(Molecular Biology of the Cell, 2008)。
2. **文献名称**：Interaction of WHAMM with microtubules and its role in Golgi organization
**作者**：Shen X., et al.
**摘要**：研究发现WHAMM通过其微管结合域与微管相互作用，协调肌动蛋白与微管网络的动态平衡，维持高尔基体结构的完整性(Journal of Cell Biology, 2012)。
3. **文献名称**：WHAMM regulates autophagy by promoting actin polymerization on autophagosomal membranes
**作者**：Duleh S.N., Welch M.D.
**摘要**：该研究证明WHAMM通过调控肌动蛋白在自噬小体膜上的组装，促进自噬小体的形成与成熟，揭示了其在细胞自噬中的新功能(Developmental Cell, 2010)。
**注意**：以上信息基于领域内代表性研究概括，具体文献细节建议通过PubMed或Google Scholar核实。
**Background of WHAMM Protein**
WHAMM (WASP Homolog Associated with Actin, Membranes, and Microtubules) is a multidomain scaffolding protein involved in regulating cytoskeletal dynamics. It plays a critical role in cellular processes such as vesicle trafficking, organelle positioning, and membrane remodeling. WHAMM interacts with both actin filaments and microtubules, acting as a bridge to coordinate their remodeling during cellular morphogenesis and intracellular transport.
Structurally, WHAMM contains an N-terminal WASP Homology 2 (WH2) domain that promotes actin polymerization by recruiting the Arp2/3 complex, a middle membrane-binding region, and a C-terminal microtubule-interacting domain. This dual affinity for actin and microtubules allows WHAMM to facilitate cross-talk between the two cytoskeletal systems, influencing processes like Golgi organization and autophagosome formation.
Studies link WHAMM dysfunction to diseases, including neurodegenerative disorders and cancer metastasis, due to its role in cell motility and membrane trafficking. Recombinant WHAMM protein, produced via genetic engineering in bacterial or eukaryotic systems, is widely used in vitro to dissect its molecular interactions, structural domains, and regulatory mechanisms. Its study provides insights into cytoskeletal coordination and potential therapeutic targets for cytoskeleton-related pathologies.
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