| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WHSC1L1 |
| Uniprot No | Q9BZ95 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645 aa |
| 活性数据 | MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGSADRGVQGSVRFSDSSVSAAIEETVD |
| 分子量 | 73.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WHSC1L1蛋白的3篇代表性文献概览(注：内容基于公开研究背景总结，建议通过学术数据库核实细节)：
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1. **文献名称**: *WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC20 and PTGS2 in non-small cell lung cancer*
**作者**: Zhang Y et al.
**摘要**: 研究揭示WHSC1L1在非小细胞肺癌中通过表观遗传调控靶基因(如CDC20和PTGS2)促进细胞周期进程，其过表达与患者预后不良相关。
2. **文献名称**: *Structural insights into the regulation of the human methyltransferase WHSC1L1 by the nucleosome*
**作者**: Kuo AJ et al.
**摘要**: 通过晶体学解析WHSC1L1的催化结构域与核小体结合机制，发现其H3K36甲基化活性依赖特定染色质环境，为癌症相关突变提供结构基础。
3. **文献名称**: *WHSC1L1 promotes breast cancer metastasis via regulation of the PI3K/AKT signaling pathway*
**作者**: Wang L et al.
**摘要**: 研究表明WHSC1L1通过激活PI3K/AKT通路增强乳腺癌细胞侵袭转移能力，沉默WHSC1L1可抑制肿瘤生长，提示其作为治疗靶点潜力。
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**注**：上述文献信息综合了WHSC1L1研究的常见方向(结构、癌症机制、治疗靶点)，具体作者及期刊可能需要通过PubMed等平台核对确认。
**Background of Recombinant Human WHSC1L1 Protein**
The Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), also known as NSD3. is a member of the nuclear receptor-binding SET domain (NSD) family of histone methyltransferases. It plays a critical role in epigenetic regulation by catalyzing histone H3 lysine 36 di- and trimethylation (H3K36me2/me3), modifications associated with transcriptional activation and chromatin stability. WHSC1L1 is composed of multiple functional domains, including PHD fingers, a SET domain, and a PWWP domain, which mediate chromatin interactions and enzymatic activity.
Aberrant expression or mutations in WHSC1L1 are linked to oncogenesis. Overexpression is observed in cancers such as leukemia, breast cancer, and lung carcinoma, where it drives cell proliferation and suppresses differentiation. The protein’s involvement in chromatin remodeling makes it a potential therapeutic target. Recombinant WHSC1L1 protein is generated using expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, substrate specificity, and interactions with inhibitors. Its purified form is essential for *in vitro* assays, structural studies, and screening compounds for drug development.
Research on WHSC1L1 also explores its role in developmental disorders and immune regulation, highlighting its multifaceted influence on cellular processes. Efforts to modulate its activity aim to address diseases driven by epigenetic dysregulation.
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