| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calpain-8, New calpain 2, nCL-2, Stomach-specific M-type calpain, CAPN8, NCL2 |
| Entrez GeneID | 388743 |
| WB Predicted band size | 79.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CAPN8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the Central region of human CAPN8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于CAPN8抗体的参考文献及其摘要概括(基于公开研究背景总结，非真实文献)：
1. **文献名称**：*"Characterization of CAPN8 in gastric mucosal protection and its interaction with CAPN9"*
**作者**：Suzuki K, et al.
**摘要**：研究利用CAPN8特异性抗体证实了CAPN8与CAPN9形成复合物(G-calpain)，在胃黏膜细胞中高表达，可能通过调节细胞凋亡参与胃癌发展。抗体用于Western blot和免疫组化分析组织分布。
2. **文献名称**：*"Role of calpain-8 in intestinal epithelial homeostasis revealed by antibody-based localization studies"*
**作者**：Adachi Y, et al.
**摘要**：通过CAPN8抗体免疫荧光染色，发现CAPN8在小鼠肠道上皮细胞中特异性表达，敲除后导致上皮屏障功能异常，提示其在肠道炎症修复中的潜在作用。
3. **文献名称**：*"Development of a novel monoclonal antibody targeting CAPN8 for colorectal cancer diagnosis"*
**作者**：Tanaka R, et al.
**摘要**：报道了一种高特异性抗CAPN8单克隆抗体的开发，验证其在结直肠癌组织中的过表达，并通过ELISA检测血清CAPN8水平，提出其作为肿瘤标志物的可能性。
(注：以上文献为示例性质，实际研究需通过PubMed等学术平台检索。)
The calpain 8 (CAPN8) antibody is a research tool designed to detect and study CAPN8. a member of the calcium-dependent cysteine protease calpain family. Calpains are involved in diverse cellular processes, including cytoskeletal remodeling, signal transduction, and apoptosis. CAPN8. also known as stomach-specific calpain, is uniquely expressed in the gastrointestinal tract, particularly in the stomach, where it plays a role in mucosal defense and digestion. Unlike other calpains, CAPN8 forms heterodimers with CAPN9 (calpain 9), and this complex is critical for maintaining gastric epithelial homeostasis and protecting against stress-induced damage.
Research on CAPN8 has linked its dysfunction to gastrointestinal pathologies, such as gastric ulcers, inflammatory bowel disease (IBD), and gastric cancer. Antibodies targeting CAPN8 enable the investigation of its expression levels, localization, and interactions in tissue samples or cell lines, aiding in understanding its regulatory mechanisms and disease associations. These antibodies are typically validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Current studies focus on CAPN8's dual role in promoting cell survival under physiological conditions while contributing to pathological processes when dysregulated. Its tissue-specific nature makes it a potential biomarker or therapeutic target for gastrointestinal disorders. However, the functional complexity of calpain family members necessitates careful interpretation of experimental results to avoid cross-reactivity or off-target effects.
×
