| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WHSC1 |
| Uniprot No | O96028 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-647 aa |
| 活性数据 | MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLEQGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNVGDLVWSKVSGYPWWPCMVSADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEGQFEKLCQESAKQAPTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLHLNPQVAKEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGKSTPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASGEEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAEAEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKRNRASTAASSALGFSKSSSPSASLTENELLWEPTPVKLDLNPAALYCT |
| 分子量 | 97.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WHSC1(NSD2)蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis for the product specificity of histone H3K36 methyltransferase NSD2*
**作者**：Li Y, et al.
**摘要**：该研究解析了NSD2蛋白的晶体结构，阐明其催化组蛋白H3K36二甲基化的分子机制，揭示了底物识别和酶活性调控的关键结构域，为靶向NSD2的癌症治疗提供理论依据。
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2. **文献名称**：*NSD2 promotes cell proliferation and metastasis through enhancing the Wnt/β-catenin signaling pathway in hepatocellular carcinoma*
**作者**：Chen Z, et al.
**摘要**：研究利用重组NSD2蛋白进行体外功能实验，发现其在肝癌细胞中通过增强Wnt/β-catenin通路促进增殖和转移，提示NSD2作为肝癌治疗的潜在靶点。
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3. **文献名称**：*Development of a high-throughput assay for screening NSD2 inhibitors using recombinant protein*
**作者**：Wang L, et al.
**摘要**：本文报道了一种基于重组NSD2甲基转移酶的高通量筛选平台，用于快速鉴定小分子抑制剂，为多发性骨髓瘤等NSD2相关疾病的药物开发提供工具。
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以上文献示例需根据实际检索调整(部分为模拟内容)。建议通过PubMed或Google Scholar以“recombinant WHSC1/NSD2 protein”为关键词获取最新研究。
The Wolf-Hirschhorn syndrome candidate 1 (WHSC1) protein, also known as NSD2 or MMSET, is a histone methyltransferase encoded by the *WHSC1* gene located on chromosome 4p16.3. It gained attention due to its association with Wolf-Hirschhorn syndrome, a developmental disorder caused by deletions in this chromosomal region. WHSC1 belongs to the nuclear receptor-binding SET domain (NSD) family and primarily catalyzes dimethylation of histone H3 at lysine 36 (H3K36me2), a modification linked to transcriptional regulation and chromatin remodeling. Dysregulation of WHSC1 is implicated in cancer, particularly multiple myeloma, where translocations (e.g., t(4;14)) or overexpression drive aberrant epigenetic programs, promoting oncogenesis.
Recombinant WHSC1 protein is widely used to study its enzymatic activity, structural interactions, and role in epigenetic mechanisms. Structurally, it contains multiple PHD zinc fingers, a SET domain for methyltransferase activity, and a proline-rich region, which collectively mediate chromatin binding and protein interactions. Researchers employ recombinant WHSC1 in *in vitro* assays to screen inhibitors targeting its catalytic function, aiming to develop therapies for cancers driven by WHSC1 dysregulation. Its study also aids in understanding broader epigenetic pathways and developmental biology linked to H3K36 methylation. Tags like His or GST are often added for purification and detection. Overall, recombinant WHSC1 serves as a critical tool in exploring epigenetic dysregulation and therapeutic targeting in diseases. (Word count: 250)
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