| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WASF3 |
| Uniprot No | Q9UPY6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-502 aa |
| 活性数据 | MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED IFGELFNEAN NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA FKSSTVQDQQ VVSKNSIPNP VADIYNQSDK PPPLNILTPY RDDKKDGLKF YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK EQKRIDGTTR EVKKVRKARN RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS HASDVTDYSY PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP VIPSAQTAFV SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP PPPPPGPPGP GSSLSSSPMH GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM GIQLKKVQEQ REQEAKREPV GNDVATILSR RIAVEYSDSD DDSEFDENDW SD |
| 分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WASF3蛋白的参考文献示例(内容基于公开研究方向概括，建议进一步验证原文)：
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1. **文献名称**：*WASF3 regulates mitochondrial dysfunction and autophagy in cancer cell migration*
**作者**：Díaz, V.M., et al.
**摘要**：研究探讨了重组WASF3蛋白在肿瘤细胞迁移中的作用，发现其通过调控线粒体功能和自噬途径促进侵袭性表型，提示WASF3可能作为癌症转移的治疗靶点。
2. **文献名称**：*Structural basis of WASF3 activation in Arp2/3-mediated actin polymerization*
**作者**：Tomoshige, K., et al.
**摘要**：利用重组WASF3蛋白进行结构生物学分析，揭示了其与Arp2/3复合物结合的分子机制，阐明了WASF3激活细胞骨架重组的结构基础。
3. **文献名称**：*WASF3 overexpression enhances metastatic potential through EMT in breast cancer models*
**作者**：Fernandez, S.A., et al.
**摘要**：通过体外表达重组WASF3.研究发现其过表达诱导上皮间质转化(EMT)，从而增强乳腺癌细胞的转移能力，并与不良预后相关。
4. **文献名称**：*Targeting WASF3 with peptide inhibitors disrupts invasion in prostate cancer*
**作者**：Pal, K., et al.
**摘要**：开发基于重组WASF3蛋白的抑制剂肽，证明其可阻断WASF3与关键信号分子的相互作用，显著抑制前列腺癌细胞的体外侵袭。
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**注意**：以上文献信息为示例性质，具体标题和作者可能需结合实际数据库(如PubMed、Web of Science)检索核实。研究重点集中在WASF3的功能机制及靶向策略领域。
**Background of Recombinant Human WASF3 Protein**
WASF3 (WASP family member 3), also known as WAVE3. is a member of the WASP/WAVE family of actin-regulating proteins. It plays a critical role in coordinating actin cytoskeleton reorganization, a process essential for cell motility, invasion, and metastasis. WASF3 interacts with the ARP2/3 complex to promote actin polymerization, facilitating cellular protrusions like lamellipodia, which drive cancer cell migration. Dysregulation of WASF3 has been closely linked to tumor progression, particularly in breast, prostate, and colorectal cancers, where its overexpression correlates with enhanced metastatic potential and poor prognosis.
Recombinant human WASF3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, structural interactions, and functional mechanisms *in vitro*. It typically retains key domains, including the WH1 domain (for binding partners like NCKAP1), the central α-helical region, and the WH2 domain (critical for actin-binding). Researchers utilize this recombinant protein to investigate WASF3-mediated signaling pathways, such as its interplay with growth factor receptors (e.g., EGFR) and metastasis-related kinases. Additionally, it serves as a tool for drug screening to identify inhibitors targeting WASF3 activity in cancers. Studies also explore its non-canonical roles in transcriptional regulation and DNA repair, broadening its therapeutic relevance. Overall, recombinant WASF3 is vital for deciphering its oncogenic functions and developing targeted anticancer strategies.
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