首页 / 产品 / 蛋白 / 其他蛋白

Recombinant Human VPS45 Protein

  • 中文名: 重组人(VPS45)蛋白
  • 别    名: h-VPS45; H1; H1VPS45; HGNC:14579; hlVps45; Leucocyte vacuolar Protein sorting 45; OTTHUMP00000014482; Vacuolar Protein sorting 45 homolog (S. cerevisiae); Vacuolar Protein sorting 45A (yeast homolog); Vacuolar Protein sorting 45A; Vacuolar Protein sorting
货号: PAX2000-12481
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点VPS45
Uniprot NoQ9NRW7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-570 aa
活性数据MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT KSFLEEVLAS GLHSRSKESS QVTSRSASRR
分子量65 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人VPS45蛋白的3篇文献摘要示例:

1. **文献名称**:*VPS45 regulates insulin signaling in diabetes through endosomal trafficking*

**作者**:Smith A, et al.

**摘要**:研究揭示了VPS45通过调控细胞内胰岛素受体的囊泡运输,影响胰岛素信号通路的完整性。实验利用重组人VPS45蛋白恢复糖尿病模型细胞中的内体分选功能,证实其改善胰岛素抵抗的作用。

2. **文献名称**:*Functional analysis of a novel VPS45 mutation in severe congenital neutropenia*

**作者**:Chen L, et al.

**摘要**:通过重组人VPS45蛋白的体外功能实验,发现其突变体导致细胞内中性粒细胞颗粒运输缺陷,阐明了VPS45突变与先天性中性粒细胞减少症的分子机制关联。

3. **文献名称**:*Recombinant VPS45 restores GLUT4 trafficking in skeletal muscle cells*

**作者**:Johnson R, et al.

**摘要**:研究证明重组人VPS45蛋白可修复肌肉细胞中GLUT4囊泡转运缺陷,提示其在代谢性疾病(如2型糖尿病)治疗中的潜在应用价值。

注:以上文献信息为模拟示例,实际文献需通过学术数据库检索获取。建议使用PubMed、Web of Science等平台,以关键词"VPS45"和"recombinant"进一步筛选。


背景信息

VPS45 (vacuolar protein sorting 45 homolog) is a member of the Sec1/Munc18 (SM) protein family, which plays a critical role in intracellular membrane trafficking processes. It functions as a regulatory scaffold to facilitate SNARE complex assembly, a key step in vesicle docking and fusion. Structurally, VPS45 contains conserved domains that interact with syntaxins and other components of the endosomal sorting machinery.

Human VPS45 is primarily involved in endosome-to-Golgi retrograde transport and endosomal maturation. It associates with the ESCRT (endosomal sorting complex required for transport) machinery to mediate cargo sorting and multivesicular body formation. Dysregulation of VPS45 has been linked to genetic disorders, including severe congenital neutropenia, where mutations impair neutrophil granule formation due to defective vesicular trafficking.

Recombinant human VPS45 protein is produced in bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. It serves as a vital tool for studying molecular mechanisms of vesicular transport, protein-protein interactions, and disease pathogenesis. Studies using recombinant VPS45 have elucidated its binding partners, such as RabGTPases and syntaxins, and its role in maintaining endosomal-lysosomal system integrity. This protein is also explored in drug screening platforms targeting trafficking-related disorders.


客户数据及评论

折叠内容

大包装询价

×