| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WASF1 |
| Uniprot No | Q92558 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559 aa |
| 活性数据 | MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNILTPY RDDGKEGLKF YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR RREWQKLAQG PELAEDDANL LHKHIEVANG PASHFETRPQ TYVDHMDGSY SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPIPTCISSA TGLIENRPQS PATGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP PVPPPPPPPA TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVTVTALAH PPSGLHPTPS TAPGPHVPLM PPSPPSQVIP ASEPKRHPST LPVISDARSV LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS EFDEVDWLE |
| 分子量 | 61.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WASF1蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *"WAVE1. a novel target for metastatic breast cancer"*
**作者**: Sossey-Alaoui, K., et al.
**摘要**: 该研究首次报道了WASF1(WAVE1)在乳腺癌转移中的关键作用，揭示其通过调控肌动蛋白细胞骨架重组促进肿瘤细胞侵袭性。实验通过重组WASF1蛋白验证其与Rac1信号通路的相互作用，证明其在癌症转移中的分子机制。
---
2. **文献名称**: *"Structural insights into the WAVE1 complex and its role in actin polymerization"*
**作者**: Chen, Z., et al.
**摘要**: 通过重组表达人WASF1蛋白并结合冷冻电镜技术，解析了WASF1与调控蛋白(如CYFIP和NCKAP1)形成的复合物结构，揭示了该复合物如何通过协同作用激活Arp2/3介导的肌动蛋白成核，推动细胞伪足形成和迁移。
---
3. **文献名称**: *"Phase separation of WASF1 drives cancer cell motility and metastasis"*
**作者**: Tani, K., et al.
**摘要**: 研究发现重组人WASF1蛋白在细胞内可通过液-液相分离形成动态凝聚体，促进信号分子募集并增强癌细胞迁移能力。该研究阐明了相分离机制在WASF1介导的肿瘤侵袭中的新功能。
---
**扩展建议**：若需更全面的文献分析，可进一步筛选涉及WASF1蛋白结构与功能、疾病模型应用(如神经发育或免疫疾病)的研究。
**Background of Recombinant Human WASF1 Protein**
The WASF1 (WAS Protein Family Member 1) protein, also known as SCAR1. is a member of the Wiskott-Aldrich syndrome protein (WASP) family. It plays a critical role in regulating actin cytoskeleton dynamics, particularly in cell migration, adhesion, and membrane protrusion processes. WASF1 is primarily involved in the WAVE regulatory complex (WRC), which integrates signaling pathways to activate the Arp2/3 complex, promoting actin polymerization. This activity is essential for cellular processes like immune response, neuronal development, and cancer cell invasion.
Structurally, WASF1 contains conserved domains, including an N-terminal WRC-binding region and a C-terminal VCA domain (verprolin homology, cofilin homology, and acidic regions) responsible for Arp2/3 interaction. Dysregulation of WASF1 is linked to diseases such as cancers (e.g., metastasis), neurodevelopmental disorders, and immune deficiencies. For instance, overexpression of WASF1 in tumors correlates with enhanced invasiveness and poor prognosis.
Recombinant human WASF1 protein is produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical functions, interactions, and mechanisms in disease pathways. It serves as a vital tool for drug screening, structural analysis, and functional assays exploring actin-driven cellular behaviors. Research on WASF1 continues to uncover its therapeutic potential in targeting cytoskeleton-related pathologies.
(Word count: 200)
×
