| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS52 |
| Uniprot No | Q8N1B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-723 aa |
| 活性数据 | AAAATMAAA ARELVLRAGT SDMEEEEGPL AGGPGLQEPL QLGELDITSD EFILDEVDVH IQANLEDELV KEALKTGVDL RHYSKQVELE LQQIEQKSIR DYIQESENIA SLHNQITACD AVLERMEQML GAFQSDLSSI SSEIRTLQEQ SGAMNIRLRN RQAVRGKLGE LVDGLVVPSA LVTAILEAPV TEPRFLEQLQ ELDAKAAAVR EQEARGTAAC ADVRGVLDRL RVKAVTKIRE FILQKIYSFR KPMTNYQIPQ TALLKYRFFY QFLLGNERAT AKEIRDEYVE TLSKIYLSYY RSYLGRLMKV QYEEVAEKDD LMGVEDTAKK GFFSKPSLRS RNTIFTLGTR GSVISPTELE APILVPHTAQ RGEQRYPFEA LFRSQHYALL DNSCREYLFI CEFFVVSGPA AHDLFHAVMG RTLSMTLKHL DSYLADCYDA IAVFLCIHIV LRFRNIAAKR DVPALDRYWE QVLALLWPRF ELILEMNVQS VRSTDPQRLG GLDTRPHYIT RRYAEFSSAL VSINQTIPNE RTMQLLGQLQ VEVENFVLRV AAEFSSRKEQ LVFLINNYDM MLGVLMERAA DDSKEVESFQ QLLNARTQEF IEELLSPPFG GLVAFVKEAE ALIERGQAER LRGEEARVTQ LIRGFGSSWK SSVESLSQDV MRSFTNFRNG TSIIQGALTQ LIQLYHRFHR VLSQPQLRAL PARAELINIH HLMVELKKHK PNF |
| 分子量 | 82.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS52蛋白的示例参考文献(文献信息为模拟示例，实际需根据真实文献调整)：
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1. **文献名称**: *Structural insights into the human VPS52 subunit and its role in the ESCRT pathway*
**作者**: Shim S, et al.
**摘要**: 该研究通过重组表达纯化人VPS52蛋白，结合冷冻电镜技术解析其三维结构，揭示了VPS52与ESCRT-III复合体的相互作用界面，并证明其在胞质分裂和内体分选中不可或缺的功能。
2. **文献名称**: *VPS52 regulates synaptic vesicle recycling via endosomal trafficking*
**作者**: Kim JH, Lee M.
**摘要**: 利用重组人VPS52体外功能实验，发现其通过与Rab GTP酶家族互作调控内体运输，影响神经元突触囊泡循环，为神经退行性疾病中VPS52突变的致病机制提供了新依据。
3. **文献名称**: *Recombinant human VPS52 rescues viral budding defects in CRISPR/Cas9-edited cells*
**作者**: Zhang Y, et al.
**摘要**: 研究证明重组人VPS52可恢复CRISPR敲除细胞中HIV-1病毒出芽能力，证实VPS52在ESCRT介导的膜脱离过程中起关键作用，为抗病毒治疗提供了潜在靶点。
4. **文献名称**: *Functional interaction between VPS52 and retromer complex in retrograde transport*
**作者**: Garcia-Perez A, et al.
**摘要**: 通过重组蛋白共沉淀实验，揭示了VPS52与逆转录复合体(retromer)之间的功能关联，表明其在细胞膜蛋白回收通路中的协同调控机制。
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**注意**：以上文献信息为虚拟示例，实际引用请通过PubMed、Google Scholar等平台检索真实发表的文献。建议使用关键词“VPS52”、“ESCRT”、“recombinant human VPS52”进行精确查询。
VPS52 (Vacuolar Protein Sorting 52), also known as SAC2 or RINT-1. is a conserved subunit of the Golgi-associated retrograde protein (GARP) complex, which plays a critical role in regulating endosome-to-Golgi retrograde transport. This protein is essential for maintaining cellular homeostasis by facilitating the tethering of endosome-derived vesicles to the trans-Golgi network (TGN), ensuring proper sorting and recycling of cargo proteins, such as lysosomal enzymes and receptors. Structurally, VPS52 interacts with other GARP components (VPS53. VPS54. and VPS51) to form a multisubunit complex that coordinates membrane trafficking pathways.
Recombinant human VPS52 protein is produced using expression systems like E. coli or mammalian cells, often tagged for purification (e.g., His-tag). Its study provides insights into mechanisms underlying intracellular trafficking, organelle dynamics, and associated disorders. Dysregulation of VPS52 has been linked to neurodegenerative diseases (e.g., Alzheimer’s), cancer progression, and developmental defects, as disrupted vesicular trafficking can impair lysosomal function, signal transduction, and cell polarity. Researchers utilize recombinant VPS52 to explore its biochemical interactions, screen for therapeutic agents targeting trafficking pathways, and model genetic mutations in vitro. Its role in cellular health and disease continues to be a focus of membrane biology and translational research.
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