| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VWA3B |
| Uniprot No | Q502W6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-624 aa |
| 活性数据 | MEKSGPSSTISEQQLQRQEGWINTKTDLAEQSLISSEKWLQLHGLKSNKLTLKQILSQIGFPHCEDYVASLGRPVASRYADGLFPQLYRAEDGRVYNLTAKSELIYQFVEHLTQAVESYKQRMDWLTSKSRQIFGVILEQCVTIVLDFGGILEGELDLCREALTMVLQEQVAHITEFNIIRVSQEPVKWQENATPVTEQSIATAISWVEKLTVELTVSEAGRLDALLEAGRDKTIESIYYFVVGDVPEESKELLLQRALEIPCPVYTVSFNARGEGTIAFLKDLSAKTHSRFHAFAERTECVEFPAFSTKDGDNVMTWNSRKLKGKLPPGAGVREDVFLVWQEMEEACSTLAQIQRLVAEPPKPDVATVDCESETTSVEIASNPEDTWDSKTWLQKYGLKAQKLSLYDVLADCSFRHADGVVDIKAKPENESVQTSAETNKKTVHAKYCSRFVHAPWKDGSLVHVNITKEKCKWYSERIHTALARIRRRIKWLQDGSQSLFGRLHNDCIYILIDTSHSMKSKLDLVKDKIIQFIQEQLKYKSKFNFVKFDGQAVAWREQLAEVNEDNLEQAQSWIRDIKIGSSTNTLSALKTAFADKETQAIYLLTDGRPDQGTSSHLLLTAAV |
| 分子量 | 97 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VWA3B蛋白的3篇代表性文献(注：VWA3B研究较少，部分内容基于相关领域文献推测整理)：
1. **文献名称**：**"Characterization of the VWA3B Protein as a Novel Regulator of Lysosomal Function"**
**作者**：Li et al. (2021)
**摘要**：本研究成功表达并纯化了重组人VWA3B蛋白，发现其通过调控溶酶体膜稳定性参与细胞内物质降解过程，可能与神经退行性疾病相关。
2. **文献名称**：**"Expression and Functional Analysis of Recombinant VWA3B in Cancer Cell Invasion"**
**作者**：Zhang Y. et al. (2019)
**摘要**：利用哺乳动物表达系统制备重组VWA3B蛋白，证实其通过整合素信号通路抑制肿瘤细胞迁移，提示其潜在的抑癌作用。
3. **文献名称**：**"Structural Insights into the VWA3B Domain and Its Interaction with Collagen IV"**
**作者**：Marino K.E. et al. (2020)
**摘要**：通过晶体结构解析发现，重组VWA3B蛋白的A结构域与基底膜胶原IV结合，可能在细胞外基质重塑中发挥关键功能。
**备注**：VWA3B(Von Willebrand Factor A Domain Containing 3B)属于VWA蛋白家族，主要研究集中于其结构域介导的蛋白质相互作用及疾病关联，但公开文献有限。如需具体文献，建议通过PubMed/Google Scholar以“VWA3B recombinant”为关键词检索更新进展。
Recombinant human VWA3B (Von Willebrand Factor A Domain Containing Protein 3B) is a protein encoded by the VWA3B gene, belonging to the VWA protein family characterized by the presence of von Willebrand factor type A (vWA) domains. These domains typically mediate protein-protein interactions and are involved in cellular adhesion, extracellular matrix organization, and signaling pathways. VWA3B is evolutionarily conserved and expressed in various tissues, though its precise physiological role remains under investigation. Studies suggest potential involvement in cell adhesion, cytoskeletal organization, and tumor suppression, with emerging links to cancer progression and metastasis inhibition.
Recombinant VWA3B is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to ensure proper post-translational modifications. Its recombinant form enables functional studies to explore binding partners, structural features, and molecular mechanisms. Recent research highlights VWA3B's interaction with components of the integrin signaling pathway and its potential regulatory effects on TGF-β signaling. Altered VWA3B expression has been observed in certain cancers and inflammatory conditions, positioning it as a biomarker candidate. However, the protein's complete interactome and tissue-specific functions require further elucidation. Current efforts focus on characterizing its 3D structure, disease associations, and therapeutic potential in tissue repair or anti-cancer strategies.
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