| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS36 |
| Uniprot No | Q86VN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386 aa |
| 活性数据 | MDRFVWTSGL LEINETLVIQ QRGVRIYDGE EKIKFDAGTL LLSTHRLIWR DQKNHECCMA ILLSQIVFIE EQAAGIGKSA KIVVHLHPAP PNKEPGPFQS SKNSYIKLSF KEHGQIEFYR RLSEEMTQRR WENMPVSQSL QTNRGPQPGR IRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN KIKDKQGDIT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA GILQVPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP LRLRVFDSGV MVIELQSHKE EEMVASALET VSEKGSLTSE EFAKLVGMSV LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQS |
| 分子量 | 43.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS36蛋白的3篇代表性文献信息(文献为虚构示例，仅用于格式演示)：
1. **文献名称**：*Crystal structure of human VPS36 reveals key ESCRT-II assembly domains*
**作者**：Langelier C et al.
**摘要**：解析了重组人VPS36蛋白的晶体结构，揭示了其与ESCRT-II复合体中其他亚基(如VPS22)结合的机制，并阐明其在多泡体形成中的关键构象变化。
2. **文献名称**：*Recombinant VPS36 regulates endosomal sorting through ubiquitin recognition*
**作者**：Hurley JH et al.
**摘要**：通过体外表达重组人VPS36蛋白，发现其GLUE结构域特异识别泛素化膜蛋白，揭示了ESCRT-II复合体在细胞内囊泡分选中的作用机制。
3. **文献名称**：*VPS36 mediates ESCRT-II interaction with HIV-1 Gag during viral budding*
**作者**：Shen QT et al.
**摘要**：研究重组VPS36蛋白与HIV-1病毒Gag蛋白的相互作用，证实其通过招募ESCRT-III Machinery促进病毒颗粒从宿主细胞膜出芽的分子机制。
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注：上述文献内容为虚构概括，实际研究中建议通过PubMed或Web of Science以“VPS36”或“ESCRT-II”为关键词检索最新文献。
**Background of Recombinant Human VPS36 Protein**
VPS36 (Vacuolar Protein Sorting 36), also known as EAP45 (ESCRT-II Associated Protein 45), is a key component of the Endosomal Sorting Complex Required for Transport-II (ESCRT-II) system. This evolutionarily conserved protein plays a critical role in membrane remodeling processes, including multivesicular body (MVB) formation, viral budding, and cytokinesis. Structurally, human VPS36 contains a GLUE (GRAM-Like Ubiquitin-binding in EAP45) domain that interacts with ubiquitinated cargo proteins and phosphatidylinositol 3-phosphate (PI3P), facilitating the sorting of ubiquitinated transmembrane proteins into intralumenal vesicles of MVBs.
The ESCRT-II complex, composed of VPS36. VPS22. and VPS25. acts as a scaffold bridging ESCRT-I and ESCRT-III machinery to coordinate membrane scission. Dysregulation of VPS36 is implicated in diseases such as cancer, neurodegeneration, and viral infections (e.g., HIV-1), where hijacking of ESCRT pathways enables viral egress.
Recombinant human VPS36 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling studies on its structural and functional roles *in vitro*. Its purification often involves affinity tags (e.g., His-tag) for ease of isolation. Research applications include elucidating ESCRT-mediated trafficking mechanisms, drug discovery targeting viral replication, and exploring cellular processes like autophagy and lysosomal degradation. Advances in recombinant VPS36 production have enhanced understanding of its ubiquitin-binding specificity and regulatory interactions within the ESCRT network.
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