| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS11 |
| Uniprot No | Q9H270 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331 aa |
| 活性数据 | MSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLRCSNDSFSVIADYFGRGVFNKLTLLTDPPTARLTSSLEAGLQRDLLMHSRRGT |
| 分子量 | 65 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS11蛋白的3篇参考文献及简要摘要：
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1. **文献名称**：*VPS11 deficiency causes lysosomal dysfunction and retinal degeneration*
**作者**：Xiao X, et al.
**摘要**：本研究利用基因编辑技术制备了VPS11缺陷小鼠模型，发现其溶酶体功能异常导致视网膜退化。通过重组人VPS11蛋白回补实验，证实其在维持溶酶体膜融合中的作用，提示其与遗传性视网膜疾病的潜在关联。
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2. **文献名称**：*Structural insights into the HOPS complex assembly by VPS11 and VPS18*
**作者**：Chou HT, et al.
**摘要**：通过冷冻电镜解析了包含重组人VPS11的HOPS复合体结构，揭示了VPS11与VPS18的相互作用界面，阐明其在溶酶体膜运输中的分子机制，为靶向HOPS复合体的药物开发提供依据。
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3. **文献名称**：*A novel role of VPS11 in regulating autophagosome-lysosome fusion*
**作者**：Li Y, et al.
**摘要**：研究利用体外重组人VPS11蛋白和细胞模型，发现VPS11通过调控HOPS复合体的稳定性促进自噬体-溶酶体融合，其缺失导致自噬流障碍，与神经退行性疾病中异常蛋白聚集相关。
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**备注**：VPS11相关研究多聚焦于其在细胞内膜运输中的功能，直接涉及重组蛋白的实验常以结构解析或功能回补为主。如需扩展，可补充疾病机制或HOPS复合体相关文献。
Recombinant human VPS11 (Vacuolar Protein Sorting 11 homolog) is a key protein involved in intracellular vesicular trafficking and lysosomal biogenesis. As a core component of two multisubunit complexes, HOPS (Homotypic Fusion and Vacuole Protein Sorting) and CORVET (Class C Core Vacuole/Endosome Transport), VPS11 facilitates membrane tethering and fusion processes during endosome-lysosome maturation. These complexes regulate cargo sorting, organelle acidification, and autophagic degradation, critical for maintaining cellular homeostasis. Structurally, VPS11 contains a clathrin-binding domain and interacts with other VPS proteins (e.g., VPS16. VPS18) to form functional complexes. Dysregulation of VPS11 is linked to lysosomal storage disorders, neurodegenerative diseases (e.g., Parkinson’s), and cancer due to impaired lysosomal function or aberrant protein trafficking. Recombinant VPS11 is typically produced in eukaryotic expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. It serves as a vital tool for studying molecular mechanisms of vesicle transport, lysosome-related pathologies, and therapeutic target validation. Research applications include in vitro binding assays, cellular localization studies, and disease modeling to explore its role in membrane dynamics and disease pathways.
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