| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS33B |
| Uniprot No | Q9H267 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-617 aa |
| 活性数据 | AFPHRPDAP ELPDFSMLKR LARDQLIYLL EQLPGKKDLF IEADLMSPLD RIANVSILKQ HEVDKLYKVE NKPALSSNEQ LCFLVRPRIK NMRYIASLVN ADKLAGRTRK YKVIFSPQKF YACEMVLEEE GIYGDVSCDE WAFSLLPLDV DLLSMELPEF FRDYFLEGDQ RWINTVAQAL HLLSTLYGPF PNCYGIGRCA KMAYELWRNL EEEEDGETKG RRPEIGHIFL LDRDVDFVTA LCSQVVYEGL VDDTFRIKCG SVDFGPEVTS SDKSLKVLLN AEDKVFNEIR NEHFSNVFGF LSQKARNLQA QYDRRRGMDI KQMKNFVSQE LKGLKQEHRL LSLHIGACES IMKKKTKQDF QELIKTEHAL LEGFNIREST SYIEEHIDRQ VSPIESLRLM CLLSITENGL IPKDYRSLKT QYLQSYGPEH LLTFSNLRRA GLLTEQAPGD TLTAVESKVS KLVTDKAAGK ITDAFSSLAK RSNFRAISKK LNLIPRVDGE YDLKVPRDMA YVFGGAYVPL SCRIIEQVLE RRSWQGLDEV VRLLNCSDFA FTDMTKEDKA SSESLRLILV VFLGGCTFSE ISALRFLGRE KGYRFIFLTT AVTNSARLME AMSEVKA |
| 分子量 | 70.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS33B蛋白的3条参考文献的简要列举：
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1. **文献名称**：*VPS33B regulates lysosomal protein trafficking and mTORC1 activity in the liver*
**作者**：Zhu Y, et al.
**摘要**：研究通过重组VPS33B蛋白及基因敲除模型，发现其通过调控溶酶体相关蛋白的分选和定位，影响mTORC1信号通路活性，揭示了其在肝脏代谢与脂质储存中的关键作用。
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2. **文献名称**：*Structural insights into the role of VPS33B in membrane tethering*
**作者**：Smith J, et al.
**摘要**：通过重组表达VPS33B蛋白并结合冷冻电镜技术，解析了其与VIPAR蛋白复合物的三维结构，揭示了其参与内体-溶酶体膜锚定过程的分子机制。
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3. **文献名称**：*VPS33B mutations disrupt vesicular trafficking and cause arthrogryposis-renal dysfunction-cholestasis syndrome*
**作者**：Gissen P, et al.
**摘要**：研究基于重组VPS33B功能实验，证实了该蛋白在细胞内运输中的核心作用，其突变导致细胞间极性异常及ARC综合征中多器官功能障碍的病理机制。
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*注：以上文献名称和内容为学术主题的合理虚构示例，实际研究需查阅真实数据库(如PubMed)。如需真实文献，建议补充具体研究背景或使用关键词检索。*
VPS33B (Vacuolar Protein Sorting 33 Homolog B) is a member of the Sec1/Munc18 (SM) protein family, which plays crucial roles in intracellular membrane trafficking and vesicle fusion. It is a component of the HOPS (Homotypic Fusion and Protein Sorting) complex, facilitating lysosomal biogenesis, endosome-lysosome trafficking, and autophagosome maturation. VPS33B interacts with other HOPS subunits, such as VPS16 and VPS18. to mediate tethering and fusion of vesicles with target organelles, ensuring proper cargo delivery and organelle function.
Mutations in the VPS33B gene are linked to arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, a rare autosomal recessive disorder characterized by liver, kidney, and neuromuscular abnormalities. Studies suggest VPS33B deficiency disrupts polarized protein sorting in epithelial cells, impairing apical-basal membrane integrity and organelle acidification.
Recombinant human VPS33B protein is produced using expression systems like bacteria or mammalian cells, enabling functional studies. It aids in dissecting molecular mechanisms of vesicle trafficking, lysosomal dysfunction, and disease pathology. Researchers utilize it to explore interactions with binding partners, screen therapeutic compounds, or model ARC syndrome in vitro. Its application extends to developing gene therapies or targeted treatments for lysosomal storage disorders and trafficking-related diseases. Ongoing research aims to clarify its role in autophagy and cellular homeostasis, offering insights into broader physiological and pathological processes.
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