| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS16 |
| Uniprot No | Q9H269 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-839 aa |
| 活性数据 | MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK |
| 分子量 | 121.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS16蛋白的**模拟参考文献示例**，供参考格式和内容方向使用(非真实文献)：
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1. **文献名称**：*Structural Insights into Human VPS16 Function in the HOPS Complex*
**作者**：Zhang, L. et al.
**摘要**：本研究利用重组表达的人VPS16蛋白，通过冷冻电镜解析其与HOPS复合体其他亚基的结合机制，揭示了VPS16在介导内体-溶酶体膜融合中的构象变化。
2. **文献名称**：*Recombinant VPS16 Facilitates Analysis of Lysosomal Trafficking Defects*
**作者**：Chen, R. & Müller, S.
**摘要**：通过体外表达重组人VPS16蛋白，发现其缺失导致溶酶体酶运输异常，证明VPS16在维持溶酶体功能中的关键作用，并建立基于重组蛋白的体外功能互补实验体系。
3. **文献名称**：*Interaction Mapping of VPS16 with CORVET Subunits Using Recombinant Assays*
**作者**：Kumar, A. et al.
**摘要**：利用重组人VPS16蛋白进行蛋白质相互作用筛选，鉴定出其与CORVET复合体核心蛋白的互作界面，为VPS16在不同膜运输复合体中的动态招募机制提供证据。
4. **文献名称**：*Disease-Associated Mutations in Recombinant VPS16 Disrupt Autophagosome Maturation*
**作者**：Tanaka, H. et al.
**摘要**：通过构建重组突变型VPS16蛋白，揭示帕金森病相关位点变异破坏其与自噬相关蛋白的结合能力，导致自噬体成熟障碍的分子机制。
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**注意**：上述文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索最新文献，关键词建议：“recombinant human VPS16”、“VPS16 HOPS complex”、“VPS16 lysosomal trafficking”。
**Background of Recombinant Human VPS16 Protein**
VPS16 (Vacuolar Protein Sorting 16) is a conserved core component of the HOPS (Homotypic Fusion and Vacuole Protein Sorting) and CORVET (Class C Core Vacuole/Endosome Tethering) complexes, which are critical for membrane trafficking and fusion events in eukaryotic cells. These complexes mediate lysosomal/vacuolar biogenesis, endosome-lysosome trafficking, and autophagy by facilitating tethering and fusion of vesicles. Human VPS16. encoded by the *VPS16* gene, acts as a scaffolding protein, stabilizing interactions within these complexes.
Recombinant human VPS16 protein is produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its production enables researchers to explore its role in intracellular trafficking, lysosomal dysfunction, and disease mechanisms, such as neurodegenerative disorders and lysosomal storage diseases. Structural studies using recombinant VPS16 help map binding domains critical for HOPS/CORVET assembly and activity. Additionally, it serves as a tool for screening modulators of membrane trafficking pathways, with potential therapeutic applications.
Dysregulation of VPS16 is linked to impaired autophagy and abnormal protein aggregation, highlighting its importance in cellular homeostasis. Recombinant VPS16 protein thus provides a vital resource for deciphering its molecular interactions and developing targeted interventions in trafficking-related pathologies.
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