| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS33A |
| Uniprot No | Q96AX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-596 aa |
| 活性数据 | MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA QYSLLKEHEV EKMFTLKGNR LPAADVKNII FFVRPRLELM DIIAENVLSE DRRGPTRDFH ILFVPRRSLL CEQRLKDLGV LGSFIHREEY SLDLIPFDGD LLSMESEGAF KECYLEGDQT SLYHAAKGLM TLQALYGTIP QIFGKGECAR QVANMMIRMK REFTGSQNSI FPVFDNLLLL DRNVDLLTPL ATQLTYEGLI DEIYGIQNSY VKLPPEKFAP KKQGDGGKDL PTEAKKLQLN SAEELYAEIR DKNFNAVGSV LSKKAKIISA AFEERHNAKT VGEIKQFVSQ LPHMQAARGS LANHTSIAEL IKDVTTSEDF FDKLTVEQEF MSGIDTDKVN NYIEDCIAQK HSLIKVLRLV CLQSVCNSGL KQKVLDYYKR EILQTYGYEH ILTLHNLEKA GLLKPQTGGR NNYPTIRKTL RLWMDDVNEQ NPTDISYVYS GYAPLSVRLA QLLSRPGWRS IEEVLRILPG PHFEERQPLP TGLQKKRQPG ENRVTLIFFL GGVTFAEIAA LRFLSQLEDG GTEYVIATTK LMNGTSWIEA LMEKPF |
| 分子量 | 67.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPS33A蛋白的3篇代表性文献及其摘要内容概括：
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1. **文献名称**: *VPS33A regulates protein sorting and lysosomal function in mammalian cells*
**作者**: Suzuki T, et al.
**摘要**: 研究通过基因编辑技术敲除VPS33A，发现其缺失导致溶酶体酶运输缺陷和自噬障碍;重组VPS33A蛋白的过表达可部分挽救表型，表明其在胞内分选及溶酶体功能中的关键作用。
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2. **文献名称**: *Structural basis for the interaction of VPS33A with HOPS complex subunits*
**作者**: Mullins C, et al.
**摘要**: 通过X射线晶体学解析重组人VPS33A蛋白与HOPS复合体亚基的结合界面，揭示其通过α螺旋结构域介导膜融合与溶酶体成熟的分子机制。
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3. **文献名称**: *VPS33A mutations in Hermansky-Pudlak syndrome impair extracellular vesicle biogenesis*
**作者**: Liu JJ, et al.
**摘要**: 在遗传病Hermansky-Pudlak综合征患者中发现VPS33A突变，重组蛋白表达实验显示突变体导致细胞外囊泡形成异常，提示VPS33A在囊泡运输中的病理相关性。
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**备注**：以上为虚拟文献示例，实际研究中建议通过PubMed或Web of Science以“VPS33A recombinant protein”为关键词检索近期论文，并结合具体研究方向筛选文献。
VPS33A (Vacuolar Protein Sorting 33 Homolog A) is a conserved component of the HOPS (Homotypic fusion and Protein Sorting) complex, which plays a pivotal role in intracellular membrane trafficking, lysosomal function, and autophagic processes. As a member of the Sec1/Munc18 (SM) protein family, VPS33A facilitates vesicle docking and fusion by interacting with SNARE proteins, ensuring proper cargo delivery to lysosomes and late endosomes. It is critical for maintaining lysosomal integrity, regulating enzyme activity, and mediating organelle acidification. Dysregulation of VPS33A is linked to severe human disorders, including arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, a rare autosomal recessive condition characterized by multisystem organ failure.
The recombinant human VPS33A protein, produced via heterologous expression systems (e.g., bacteria, yeast, or mammalian cells), enables detailed biochemical and structural studies to unravel its molecular mechanisms. Researchers utilize it to investigate interactions with HOPS complex subunits, SNARE proteins, or disease-associated mutants. Recombinant VPS33A also serves as a tool for drug screening aimed at correcting trafficking defects in ARC syndrome or other lysosomal storage disorders. Its study contributes to understanding broader pathways in cellular homeostasis, membrane repair, and autophagy, offering potential therapeutic targets for neurodegenerative diseases, cancer, and metabolic syndromes linked to lysosomal dysfunction.
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