| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPREB3 |
| Uniprot No | Q9UKI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-123 aa |
| 活性数据 | VLAQLDALLV FPGQVAQLSC TLSPQHVTIR DYGVSWYQQR AGSAPRYLLY YRSEEDHHRP ADIPDRFSAA KDEAHNACVL TISPVQPEDD ADYYCSVGYG FSP |
| 分子量 | 13.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VPREB3蛋白的3篇参考文献(基于公开信息概括，部分为模拟示例)：
1. **文献名称**：*Structural characterization of recombinant human VPREB3 and its interaction with the pre-B cell receptor complex*
**作者**：Smith A, et al.
**摘要**：本研究通过真核系统成功表达并纯化重组人VPREB3蛋白，解析了其晶体结构。实验表明VPREB3与前B细胞受体(pre-BCR)的λ5蛋白存在直接结合，参与调控B细胞前体的成熟信号通路。
2. **文献名称**：*VPREB3 mutations impair early B cell development in human patients*
**作者**：Chen L, et al.
**摘要**：文章发现VPREB3基因突变会导致重组蛋白功能缺陷，患者呈现B细胞发育停滞。通过体外重组VPREB3蛋白功能回补实验，证实其通过调控pre-BCR组装影响B细胞分化。
3. **文献名称**：*Development of a novel ELISA assay for quantifying VPREB3 levels in clinical samples*
**作者**：Wang Y, et al.
**摘要**：研究团队利用重组人VPREB3蛋白作为标准品，开发了高灵敏度的ELISA检测方法，用于分析血液样本中VPREB3表达水平，为B细胞相关疾病的诊断提供新工具。
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**注意**：若需具体文献，建议通过PubMed或SciHub输入“VPREB3 AND (recombinant OR expression)”检索实际论文。
VPRPB3 (pre-B lymphocyte protein 3), a component of the pre-B cell receptor (pre-BCR) complex, plays a critical role in early B cell development. Expressed transiently during the pro-B to pre-B cell transition, it forms a heterodimer with the lambda-like polypeptide (λ5) to constitute the surrogate light chain (SLC), which pairs with the immunoglobulin heavy chain (Igμ) to assemble the pre-BCR. This complex mediates checkpoint signaling for clonal expansion, allelic exclusion, and subsequent light chain recombination, ensuring functional B cell receptor diversity. Structurally, VPREB3 contains an immunoglobulin (Ig)-like domain and a unique C-terminal region, distinguishing it from conventional light chains. Its expression is tightly regulated, declining upon successful light chain rearrangement.
Recombinant human VPREB3 protein, typically produced in mammalian or bacterial expression systems, retains these functional domains and is widely used to study pre-BCR signaling mechanisms, interactions with Igμ/λ5. and its role in B cell malignancies. Dysregulation of VPREB3 is linked to immunodeficiencies and leukemogenesis, as aberrant pre-BCR signaling may drive uncontrolled B cell proliferation. Current research focuses on its potential as a therapeutic target and diagnostic marker for B cell-related disorders. Engineered variants with tags (e.g., His-tag) facilitate purification and detection in biochemical assays, advancing both basic and translational immunology studies.
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