| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKIL |
| Uniprot No | P12757 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-684aa |
| 氨基酸序列 | MENLQTNFSL VQGSTKKLNG MGDDGSPPAK KMITDIHANG KTINKVPTVK KEHLDDYGEA PVETDGEHVK RTCTSVPETL HLNPSLKHTL AQFHLSSQSS LGGPAAFSAR HSQESMSPTV FLPLPSPQVL PGPLLIPSDS STELTQTVLE GESISCFQVG GEKRLCLPQV LNSVLREFTL QQINTVCDEL YIYCSRCTSD QLHILKVLGI LPFNAPSCGL ITLTDAQRLC NALLRPRTFP QNGSVLPAKS SLAQLKETGS AFEVEHECLG KCQGLFAPQF YVQPDAPCIQ CLECCGMFAP QTFVMHSHRS PDKRTCHWGF ESAKWHCYLH VNQKYLGTPE EKKLKIILEE MKEKFSMRSG KRNQSKTDAP SGMELQSWYP VIKQEGDHVS QTHSFLHPSY YLYMCDKVVA PNVSLTSAVS QSKELTKTEA SKSISRQSEK AHSSGKLQKT VSYPDVSLEE QEKMDLKTSR ELCSRLDASI SNNSTSKRKS ESATCNLVRD INKVGIGLVA AASSPLLVKD VICEDDKGKI MEEVMRTYLK QQEKLNLILQ KKQQLQMEVK MLSSSKSMKE LTEEQQNLQK ELESLQNEHA QRMEEFYVEQ KDLEKKLEQI MKQKCTCDSN LEKDKEAEYA GQLAELRQRL DHAEADRQEL QDELRQEREA RQKLEMMIKE LKLQILKSSK TAKE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3篇关于SKIL(SnoN)重组蛋白的参考文献示例(注：非真实文献，仅供格式参考)：
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1. **文献名称**：*Expression and functional characterization of recombinant SKIL protein in TGF-β signaling regulation*
**作者**：Chen L, et al.
**摘要**：本研究通过在大肠杆菌系统中表达并纯化重组人源SKIL蛋白，验证其通过结合Smad蛋白抑制TGF-β信号通路的活性，为靶向该通路的药物开发提供了实验基础。
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2. **文献名称**：*Structural analysis of SKIL/SnoN recombinant protein reveals key domains for transcriptional repression*
**作者**：Kimura T, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度SKIL重组蛋白，结合X射线晶体学解析其三维结构，发现其N端结构域是结合Smad4并抑制TGF-β依赖性基因转录的关键区域。
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3. **文献名称**：*Recombinant SKIL fusion protein enhances wound healing by modulating TGF-β1 activity in a murine model*
**作者**：Zhang Y, et al.
**摘要**：构建SKIL-EGF融合蛋白并在哺乳动物细胞中表达，证明其通过拮抗TGF-β1的促纤维化作用，显著加速小鼠皮肤创面愈合，提示其在组织修复中的潜在治疗价值。
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注：以上文献为模拟生成，实际研究中建议通过PubMed或Web of Science以“SKIL recombinant protein”或“SnoN recombinant”为关键词检索真实文献。
**Background of SKIL Recombinant Protein**
SKIL (also known as SnoN) is a member of the Ski family of transcriptional regulators, playing a critical role in modulating transforming growth factor-beta (TGF-β) signaling pathways. TGF-β regulates diverse cellular processes, including proliferation, differentiation, apoptosis, and immune responses. SKIL acts as a negative regulator by binding to Smad proteins (e.g., Smad2/3/4), disrupting their ability to form transcriptionally active complexes, thereby fine-tuning TGF-β-mediated gene expression. Dysregulation of SKIL has been implicated in diseases such as cancer, fibrosis, and autoimmune disorders, where aberrant TGF-β signaling contributes to pathogenesis.
Recombinant SKIL protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and functional roles. Typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains key functional domains, such as the Smad-binding region, enabling researchers to explore its mechanisms in TGF-β pathway inhibition. Its applications span from biochemical assays (e.g., protein-protein interaction studies) to cell-based experiments assessing its effects on proliferation, migration, or differentiation.
In cancer research, SKIL’s dual role—acting as both an oncogene and tumor suppressor in context-dependent manner—has spurred interest in targeting it for therapeutic intervention. Recombinant SKIL aids in drug screening, mechanistic studies, and biomarker discovery. Additionally, its involvement in fibrosis highlights potential applications in developing antifibrotic therapies. Challenges include optimizing protein stability and post-translational modification fidelity, which influence functional consistency.
Overall, SKIL recombinant protein serves as a vital tool for unraveling TGF-β signaling complexities and advancing therapeutic strategies for related diseases.
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