| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SHC2 |
| Uniprot No | P98077 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582aa |
| 氨基酸序列 | MTQGPGGRAP PAPPAPPEPE APTTFCALLP RMPQWKFAAP GGFLGRGPAA ARAAGASGGA DPQPEPAGPG GVPALAAAVL GACEPRCAAP CPLPALSRCR GAGSRGSRGG RGAAGSGDAA AAAEWIRKGS FIHKPAHGWL HPDARVLGPG VSYVVRYMGC IEVLRSMRSL DFNTRTQVTR EAINRLHEAV PGVRGSWKKK APNKALASVL GKSNLRFAGM SISIHISTDG LSLSVPATRQ VIANHHMPSI SFASGGDTDM TDYVAYVAKD PINQRACHIL ECCEGLAQSI ISTVGQAFEL RFKQYLHSPP KVALPPERLA GPEESAWGDE EDSLEHNYYN SIPGKEPPLG GLVDSRLALT QPCALTALDQ GPSPSLRDAC SLPWDVGSTG TAPPGDGYVQ ADARGPPDHE EHLYVNTQGL DAPEPEDSPK KDLFDMRPFE DALKLHECSV AAGVTAAPLP LEDQWPSPPT RRAPVAPTEE QLRQEPWYHG RMSRRAAERM LRADGDFLVR DSVTNPGQYV LTGMHAGQPK HLLLVDPEGV VRTKDVLFES ISHLIDHHLQ NGQPIVAAES ELHLRGVVSR EP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SHC2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Molecular Cloning and Functional Analysis of SHC2 in Neural Development*
**作者**：Li, Y. et al.
**摘要**：研究报道了SHC2重组蛋白的克隆及体外表达，发现其通过PTB结构域与TrkA受体结合，激活MAPK通路，促进神经元分化，提示SHC2在神经营养信号传导中的关键作用。
2. **文献名称**：*SHC2 Regulates ErbB Receptor Signaling in Breast Cancer Cells*
**作者**：Müller, J. & Schmidt, A.
**摘要**：通过纯化SHC2重组蛋白，揭示其与ErbB3受体的特异性结合，并增强PI3K-AKT通路活性，促进乳腺癌细胞迁移，为靶向SHC2的癌症治疗提供依据。
3. **文献名称**：*Structural Characterization of SHC2 Phosphorylation Sites*
**作者**：Chen, X. et al.
**摘要**：利用重组SHC2蛋白进行质谱分析，鉴定其Tyr残基磷酸化位点，证实这些位点调控SHC2与Grb2的相互作用，影响下游EGFR信号通路的激活效率。
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注：上述文献为示例，实际研究需根据具体数据库检索。若文献不足，建议扩展关键词(如“Shc family”或“信号适配蛋白”)进行查找。
SHC2 (SHC-transforming protein 2), also known as SCK or SHC-A, is an adaptor protein belonging to the SHC family, which plays a critical role in intracellular signaling transduction. Structurally, it contains a phosphotyrosine-binding (PTB) domain and a Src homology 2 (SH2) domain, enabling interactions with phosphorylated tyrosine residues on activated receptor tyrosine kinases (RTKs) and downstream signaling molecules. SHC2 facilitates signal propagation through the Ras-MAPK pathway, influencing cellular processes including proliferation, differentiation, and apoptosis.
Recombinant SHC2 protein is engineered using genetic engineering techniques, typically expressed in bacterial (e.g., E. coli) or mammalian systems. Its production allows controlled studies of SHC2's molecular interactions and signaling mechanisms in vitro. Research has linked SHC2 to neurotrophic signaling pathways, particularly through its association with Trk receptors, suggesting roles in neuronal survival and synaptic plasticity. Dysregulation of SHC2 has been implicated in cancer progression and neurodegenerative disorders, making it a potential therapeutic target or biomarker.
The recombinant form is widely used to investigate RTK-dependent signaling cascades, protein-protein interactions, and phosphorylation events. Challenges in its application include maintaining proper folding and post-translational modifications, which often necessitate mammalian expression systems. Current studies focus on deciphering its tissue-specific functions and crosstalk with other adaptor proteins like Grb2. As a research tool, recombinant SHC2 contributes to understanding cellular signaling pathologies and drug discovery efforts targeting RTK-related diseases.
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