| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SESN3 |
| Uniprot No | P58005 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 氨基酸序列 | MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF |
| 预测分子量 | 40.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SESN3(Sestrin3)重组蛋白的参考文献示例(注：文献信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Recombinant Sestrin3 Protein Attenuates Oxidative Stress via Nrf2 Signaling in Hepatocytes"*
**作者**: Kim, J. et al.
**摘要**: 研究报道了在大肠杆菌中表达并纯化重组人SESN3蛋白，验证其通过激活Nrf2通路减轻肝细胞氧化损伤的功能，为代谢性疾病治疗提供潜在策略。
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2. **文献名称**: *"Structural and Functional Characterization of Recombinant Human Sestrin3"*
**作者**: Chen, L. et al.
**摘要**: 通过哺乳动物细胞系统表达SESN3重组蛋白，结合X射线晶体学解析其三维结构，揭示了SESN3与mTORC1结合的活性位点，阐明其在代谢调控中的作用机制。
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3. **文献名称**: *"Recombinant Sestrin3 Ameliorates Insulin Resistance in Diabetic Mouse Models"*
**作者**: Smith, R. et al.
**摘要**: 利用重组SESN3蛋白进行动物实验，证明其通过抑制mTORC1通路改善糖尿病小鼠的胰岛素敏感性，提示其作为糖尿病治疗候选分子的潜力。
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4. **文献名称**: *"Purification and In Vitro Activity Analysis of Sestrin3 Recombinant Protein"*
**作者**: Wang, Y. et al.
**摘要**: 优化了SESN3在昆虫细胞-杆状病毒系统中的表达和纯化流程，并通过体外实验验证其调控AMPK/mTOR信号通路的活性，为靶向药物开发提供基础数据。
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**建议**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台以关键词“Sestrin3 recombinant protein”或“SESN3 expression”检索获取。部分研究可能聚焦于SESN3的基因功能而非重组蛋白，建议筛选时注意研究主题。
SESN3 (Sestrin 3) is a member of the sestrin family of stress-responsive proteins, evolutionarily conserved from invertebrates to humans. It plays a critical role in regulating cellular homeostasis under various stress conditions, particularly oxidative and genotoxic stress. SESN3 is involved in modulating metabolic pathways, including the AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) signaling axes, thereby influencing energy balance, autophagy, and antioxidant defense mechanisms. Its expression is induced by stress-activated transcription factors such as p53 and forkhead box O (FOXO), linking it to aging, metabolic disorders, and cancer progression.
Structurally, SESN3 contains a conserved sestrin domain that enables its redox-regulatory functions. Recombinant SESN3 protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein retains functional properties, including the ability to interact with AMPK and regulate mTORC1 activity, making it a valuable tool for *in vitro* studies. Researchers utilize recombinant SESN3 to investigate its role in mitigating oxidative damage, enhancing stress resistance, and modulating metabolic pathways in cellular models. Additionally, it has potential therapeutic applications in metabolic syndromes, neurodegenerative diseases, and cancer, where dysregulation of stress-response pathways is a hallmark. Ongoing studies aim to elucidate its structural nuances and develop SESN3-based interventions for diseases linked to oxidative stress and metabolic imbalance.
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