| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP11B2 |
| Uniprot No | P19099 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-503aa |
| 氨基酸序列 | GTRAARAPRTVLPFEAMPQHPGNRWLRLLQIWREQGYEHLHLEMHQTFQE LGPIFRYNLGGPRMVCVMLPEDVEKLQQVDSLHPCRMILEPWVAYRQHRG HKCGVFLLNGPEWRFNRLRLNPDVLSPKAVQRFLPMVDAVARDFSQALKK KVLQNARGSLTLDVQPSIFHYTIEASNLALFGERLGLVGHSPSSASLNFL HALEVMFKSTVQLMFMPRSLSRWISPKVWKEHFEAWDCIFQYGDNCIQKI YQELAFNRPQHYTGIVAELLLKAELSLEAIKANSMELTAGSVDTTAFPLL MTLFELARNPDVQQILRQESLAAAASISEHPQKATTELPLLRAALKETLR LYPVGLFLERVVSSDLVLQNYHIPAGTLVQVFLYSLGRNAALFPRPERYN PQRWLDIRGSGRNFHHVPFGFGMRQCLGRRLAEAEMLLLLHHVLKHFLVE TLTQEDIKMVYSFILRPGTSPLLTFRAIN |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP11B2重组蛋白的3篇参考文献示例(内容基于公开研究整理，部分信息可能需要核实)：
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1. **标题**: "Functional Expression and Characterization of Human Aldosterone Synthase (CYP11B2) in Escherichia coli"
**作者**: Zhang H. et al.
**摘要**: 研究报道了利用大肠杆菌系统成功表达人源CYP11B2重组蛋白，优化了血红素辅因子结合条件，并通过体外酶活实验验证其催化皮质酮转化为醛固酮的能力，为高通量抑制剂筛选提供了平台。
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2. **标题**: "Structural Insights into Aldosterone Synthase through Recombinant Protein Crystallography"
**作者**: Petruk A.A. et al.
**摘要**: 通过昆虫细胞表达系统获得高纯度CYP11B2重组蛋白，解析其晶体结构并揭示关键底物结合域，为靶向治疗原发性醛固酮增多症的抑制剂设计提供了结构基础。
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3. **标题**: "Development of a Yeast-Based Assay for Human CYP11B2 Activity Using Recombinant Protein Expression"
**作者**: Gomez-Sanchez C.E. & Zhou M.Y.
**摘要**: 在酵母中表达功能性CYP11B2重组蛋白，建立醛固酮合成活性检测体系，用于评估基因突变对酶功能的影响及药物相互作用研究。
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如需具体文献，建议通过PubMed或Web of Science以关键词“CYP11B2 recombinant expression”或“aldosterone synthase purification”检索最新论文。
CYP11B2. also known as aldosterone synthase, is a cytochrome P450 enzyme encoded by the CYP11B2 gene in humans. It plays a critical role in the synthesis of aldosterone, a hormone essential for regulating blood pressure and electrolyte balance. Located in the mitochondrial membrane of adrenal zona glomerulosa cells, CYP11B2 catalyzes the final steps of aldosterone biosynthesis: the conversion of 11-deoxycorticosterone to corticosterone, and subsequently to 18-hydroxycorticosterone and aldosterone via hydroxylation and oxidation reactions. Its activity is tightly regulated by the renin-angiotensin-aldosterone system (RAAS), potassium levels, and adrenocorticotropic hormone (ACTH).
Recombinant CYP11B2 protein is produced using genetic engineering techniques, often expressed in heterologous systems like Escherichia coli, yeast, or mammalian cells. This allows researchers to obtain purified, functional enzyme for structural and functional studies. Recombinant CYP11B2 is particularly valuable for investigating aldosterone dysregulation linked to hypertension, heart failure, and primary aldosteronism. It also serves as a target for drug discovery, aiding in the development of selective inhibitors to treat conditions caused by excessive aldosterone production.
Structurally, CYP11B2 shares high homology with CYP11B1 (involved in cortisol synthesis), but differences in substrate specificity and regulation make selective inhibition crucial. Recombinant protein studies have clarified mechanisms of catalytic activity, substrate binding, and interactions with redox partners like adrenodoxin. Additionally, mutations in CYP11B2 associated with familial hyperaldosteronism or aldosterone deficiency can be functionally characterized using recombinant variants. Its recombinant form has become indispensable for both basic research and therapeutic innovation in endocrine disorders.
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