| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SHC3 |
| Uniprot No | Q92529 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MLPRTKYNRF RNDSVTSVDD LLHSLSVSGG GGKVSAARAT PAAAPYLVSG EALRKAPDDG PGSLGHLLHK VSHLKLSSSG LRGLSSAARE RAGARLSGSC SAPSLAAPDG SAPSAPRAPA MSAARKGRPG DEPLPRPPRG APHASDQVLG PGVTYVVKYL GCIEVLRSMR SLDFSTRTQI TREAISRVCE AVPGAKGAFK KRKPPSKMLS SILGKSNLQF AGMSISLTIS TASLNLRTPD SKQIIANHHM RSISFASGGD PDTTDYVAYV AKDPVNRRAC HILECCDGLA QDVIGSIGQA FELRFKQYLQ CPTKIPALHD RMQSLDEPWT EEEGDGSDHP YYNSIPSKMP PPGGFLDTRL KPRPHAPDTA QFAGKEQTYY QGRHLGDTFG EDWQQTPLRQ GSSDIYSTPE GKLHVAPTGE APTYVNTQQI PPQAWPAAVS SAESSPRKDL FDMKPFEDAL KNQPLGPVLS KAASVECISP VSPRAPDAKM LEELQAETWY QGEMSRKEAE GLLEKDGDFL VRKSTTNPGS FVLTGMHNGQ AKHLLLVDPE GTIRTKDRVF DSISHLINHH LESSLPIVSA GSELCLQQPV ERKQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SHC3重组蛋白的3篇参考文献及其摘要概括(部分信息为示例性内容，具体文献需根据实际检索结果调整)：
1. **标题**: "Functional characterization of recombinant SHC3 protein in neuronal signaling pathways"
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了人源SHC3重组蛋白，验证其与TrkA受体的结合能力，证明SHC3在神经营养因子信号转导中通过激活MAPK通路调控神经元分化。
2. **标题**: "Structural insights into the SHC3 adaptor protein by recombinant expression and crystallography"
**作者**: Tanaka K, et al.
**摘要**: 利用昆虫细胞表达系统获得高纯度SHC3重组蛋白，解析其PTB结构域的晶体结构，揭示其特异性识别磷酸化酪氨酸位点的分子机制，为癌症靶点研究提供结构基础。
3. **标题**: "SHC3 recombinant protein enhances oncogenic signaling in glioblastoma cells"
**作者**: Patel R, et al.
**摘要**: 通过哺乳动物细胞表达SHC3重组蛋白，发现其过表达可增强EGFR下游的PI3K/AKT通路活性，促进胶质母细胞瘤细胞侵袭，提示SHC3作为潜在治疗靶点。
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注：以上为示例性内容，实际文献需通过PubMed、Web of Science等平台检索关键词(如"SHC3 recombinant"、"SHC3 expression")获取。若研究较少，可扩展至SHC家族蛋白或SHC3基因功能相关文献。
**Background of SHC3 Recombinant Protein**
SHC3 (Src homology 2 domain-containing transforming protein C3) is a member of the SHC family of adaptor proteins, which play critical roles in intracellular signaling pathways. These proteins are characterized by their SH2 domain, which enables interaction with phosphorylated tyrosine residues on activated receptor tyrosine kinases (RTKs) or other signaling molecules. SHC3. encoded by the *SHC3* gene, is particularly noted for its involvement in transmitting signals from growth factor receptors to downstream effectors, such as the Ras/MAPK pathway, regulating processes like cell proliferation, differentiation, and survival.
Unlike other SHC isoforms (e.g., SHC1/p66Shc), SHC3 exhibits tissue-specific expression, with higher levels observed in the brain and nervous system. It has been implicated in neural development, synaptic plasticity, and neuroprotection. Dysregulation of SHC3 is associated with neurological disorders, including Alzheimer’s disease, and certain cancers, where it may influence tumor progression or metastasis.
Recombinant SHC3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. This protein retains key domains, such as the SH2 and phosphotyrosine-binding (PTB) domains, enabling studies on its interactions with RTKs (e.g., Trk receptors) or downstream signaling components. Researchers utilize SHC3 recombinant protein to investigate its role in signaling cascades, screen for therapeutic agents targeting SHC3-mediated pathways, or explore its diagnostic potential in diseases. Its production often includes tags (e.g., His-tag) for ease of purification and detection.
Overall, SHC3 recombinant protein serves as a vital tool for dissecting the molecular mechanisms of SHC3 in health and disease, offering insights into targeted therapies for neurological and oncological conditions.
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