| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3BP2 |
| Uniprot No | P78314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MAAEEMHWPV PMKAIGAQNL LTMPGGVAKA GYLHKKGGTQ LQLLKWPLRF VIIHKRCVYY FKSSTSASPQ GAFSLSGYNR VMRAAEETTS NNVFPFKIIH ISKKHRTWFF SASSEEERKS WMALLRREIG HFHEKKDLPL DTSDSSSDTD SFYGAVERPV DISLSPYPTD NEDYEHDDED DSYLEPDSPE PGRLEDALMH PPAYPPPPVP TPRKPAFSDM PRAHSFTSKG PGPLLPPPPP KHGLPDVGLA AEDSKRDPLC PRRAEPCPRV PATPRRMSDP PLSTMPTAPG LRKPPCFRES ASPSPEPWTP GHGACSTSSA AIMATATSRN CDKLKSFHLS PRGPPTSEPP PVPANKPKFL KIAEEDPPRE AAMPGLFVPP VAPRPPALKL PVPEAMARPA VLPRPEKPQL PHLQRSPPDG QSFRSFSFEK PRQPSQADTG GDDSDEDYEK VPLPNSVFVN TTESCEVERL FKATSPRGEP QDGLYCIRNS STKSGKVLVV WDETSNKVRN YRIFEKDSKF YLEGEVLFVS VGSMVEHYHT HVLPSHQSLL LRHPYGYTGP R |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3BP2重组蛋白的3篇参考文献示例(内容基于典型研究方向，供参考)：
1. **标题**：SH3BP2 regulates osteoclastogenesis via NF-κB and MAPK signaling pathways
**作者**：Y. Yoshida et al.
**摘要**：该研究利用重组SH3BP2蛋白分析其在破骨细胞分化中的作用，发现其通过激活NF-κB和MAPK通路促进破骨细胞生成，突变会导致信号异常并与遗传性骨疾病相关。
2. **标题**：SH3BP2 mutations induce TNF-α overproduction in cherubism through autoinflammatory mechanisms
**作者**：M. Mukai et al.
**摘要**：通过重组SH3BP2蛋白的体外实验，发现其突变导致巨噬细胞中TNF-α异常分泌，揭示了导致颌骨破坏性炎症(家族性巨颌症)的自发炎症机制。
3. **标题**：Structural analysis of SH3BP2 reveals a critical role of its PH domain in B-cell receptor signaling
**作者**：K. Le et al.
**摘要**：研究通过重组SH3BP2蛋白的晶体结构解析，阐明其PH结构域与细胞膜磷脂的结合能力，并证明其对B细胞受体信号传导的关键调控作用。
(注：以上文献为示例，实际引用需以具体论文为准。)
**Background of SH3BP2 Recombinant Protein**
SH3BP2 (SH3 domain-binding protein 2), also known as 3BP2. is an adaptor protein involved in intracellular signaling pathways regulating immune responses, bone homeostasis, and oncogenesis. It was initially identified through its interaction with the SH3 domain of Abl tyrosine kinase. Structurally, SH3BP2 contains an N-terminal pleckstrin homology (PH) domain, a central proline-rich region with SH3-binding motifs, and a C-terminal immunoreceptor tyrosine-based activation motif (ITAM). These domains enable interactions with kinases (e.g., SYK, SRC), adaptors, and membrane receptors, modulating signaling cascades such as NF-κB, MAPK, and PI3K/AKT.
Dysregulation of SH3BP2 is linked to human diseases. Gain-of-function mutations cause cherubism, a rare genetic disorder characterized by jawbone destruction. SH3BP2 also interacts with receptors like CD28 and TLRs, implicating it in autoimmune diseases (e.g., rheumatoid arthritis) and cancer progression via abnormal immune cell activation or tumor microenvironment modulation.
Recombinant SH3BP2 proteins are engineered for research to study its biochemical functions, interactions, and therapeutic targeting. Produced in bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins retain functional domains and post-translational modifications critical for activity. Applications include *in vitro* binding assays, structural studies, and screening for inhibitors. Recombinant SH3BP2 has also been used to investigate its role in osteoclastogenesis, B-cell/T-cell signaling, and pathological mechanisms in cherubism models. Its versatility makes it a valuable tool for deciphering signaling networks and developing targeted therapies for related disorders.
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