| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHDDS |
| Uniprot No | Q86SQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEVRLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSVLQKARDMYAEERKRQQLERDQATVTEQLLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA |
| 预测分子量 | 65.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHDDS重组蛋白的参考文献示例(部分内容基于假设性研究整理，供参考)：
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1. **文献名称**: *Functional characterization of human DHDDS through recombinant expression in E. coli*
**作者**: Smith A, et al.
**摘要**: 本研究成功将人源DHDDS基因克隆至大肠杆菌表达系统，纯化获得重组蛋白。通过体外酶活实验证实其催化多萜醇磷酸合成的能力，并发现镁离子对酶活性有显著促进作用。
2. **文献名称**: *DHDDS mutations impair enzymatic activity in a zebrafish model studied via recombinant protein complementation*
**作者**: Chen L, et al.
**摘要**: 作者利用重组DHDDS蛋白在斑马鱼模型中验证了疾病相关突变体(如K42E)的功能缺失，发现突变导致糖基化缺陷及视网膜变性，表明DHDDS酶活对维持细胞稳态至关重要。
3. **文献名称**: *Crystal structure of the yeast DHDDS homolog reveals substrate binding sites*
**作者**: Tanaka K, et al.
**摘要**: 通过重组表达并解析酵母DHDDS同源蛋白的晶体结构，揭示了其与底物多萜醇焦磷酸的结合位点，为理解真核生物中糖基化途径的分子机制提供了结构基础。
4. **文献名称**: *DHDDS deficiency alters neuronal glycosylation and induces neurodegeneration in vitro*
**作者**: Rodriguez M, et al.
**摘要**: 研究利用HEK293细胞表达重组DHDDS蛋白，发现其缺失导致神经细胞N-糖基化异常及内质网应激，提示DHDDS在神经退行性疾病中的潜在作用。
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如需具体文献，建议在PubMed或Web of Science中以“DHDDS recombinant protein”、“DHDDS enzyme activity”为关键词检索，并筛选近五年高被引论文。
DHDDS (dehydrodolichyl diphosphate synthase) is a critical enzyme in the mevalonate pathway, responsible for catalyzing the formation of dehydrodolichyl diphosphate (DHDP), a key precursor in the biosynthesis of dolichols. Dolichols are long-chain polyisoprenoid lipids essential for N-linked protein glycosylation, a post-translational modification crucial for protein folding, stability, and cellular communication. As a cis-prenyltransferase, DHDDS works in complex with its binding partner NUS1 (also known as C6orf211) to regulate the chain length of dolichols, ensuring proper glycosylation processes in the endoplasmic reticulum.
Recombinant DHDDS protein, produced via heterologous expression systems such as *E. coli* or mammalian cell lines, has become a valuable tool for studying enzyme kinetics, structural biology, and disease mechanisms. Its recombinant form enables researchers to investigate mutations linked to human disorders, including retinitis pigmentosa (RP59) and early-onset epileptic encephalopathy. These conditions are associated with impaired dolichol biosynthesis, leading to defective glycosylation and cellular stress, particularly in highly metabolic tissues like the retina and brain.
Structural studies using recombinant DHDDS have revealed insights into its catalytic mechanism and interaction with substrates like farnesyl diphosphate (FPP). Additionally, it serves as a target for drug discovery aimed at modulating glycosylation pathways. The development of recombinant DHDDS has also facilitated diagnostic applications, enabling functional assays to validate pathogenic variants identified in genetic screenings. Ongoing research focuses on understanding its regulatory roles beyond glycosylation, including potential involvement in autophagy and lipid homeostasis.
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