| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBXIP1 |
| Uniprot No | Q96AQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-731 aa |
| 活性数据 | MASCPDSDNS WVLAGSESLP VETLGPASRM DPESERALQA PHSPSKTDGK ELAGTMDGEG TLFQTESPQS GSILTEETEV KGTLEGDVCG VEPPGPGDTV VQGDLQETTV VTGLGPDTQD LEGQSPPQSL PSTPKAAWIR EEGRCSSSDD DTDVDMEGLR RRRGREAGPP QPMVPLAVEN QAGGEGAGGE LGISLNMCLL GALVLLGLGV LLFSGGLSES ETGPMEEVER QVLPDPEVLE AVGDRQDGLR EQLQAPVPPD SVPSLQNMGL LLDKLAKENQ DIRLLQAQLQ AQKEELQSLM HQPKGLEEEN AQLRGALQQG EAFQRALESE LQQLRARLQG LEADCVRGPD GVCLSGGRGP QGDKAIREQG PREQEPELSF LKQKEQLEAE AQALRQELER QRRLLGSVQQ DLERSLQDAS RGDPAHAGLA ELGHRLAQKL QGLENWGQDP GVSANASKAW HQKSHFQNSR EWSGKEKWWD GQRDRKAEHW KHKKEESGRE RKKNWGGQED REPAGRWKEG RPRVEESGSK KEGKRQGPKE PPRKSGSFHS SGEKQKQPRW REGTKDSHDP LPSWAELLRP KYRAPQGCSG VDECARQEGL TFFGTELAPV RQQELASLLR TYLARLPWAG QLTKELPLSP AFFGEDGIFR HDRLRFRDFV DALEDSLEEV AVQQTGDDDE VDDFEDFIFS HFFGDKALKK RSGKKDKHSQ SPRAAGPREG HSHSHHHHHR G |
| 分子量 | 80.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PBXIP1(HPIP)蛋白的参考文献摘要示例：
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1. **文献名称**: *HPIP promotes tumor growth through Wnt/β-catenin signaling in breast cancer*
**作者**: Zhang Y et al.
**摘要**: 本研究通过重组HPIP蛋白发现其通过稳定β-catenin蛋白，增强Wnt信号通路活性，从而促进乳腺癌细胞增殖。实验表明，HPIP敲低可抑制肿瘤生长，提示其作为治疗靶点的潜力。
2. **文献名称**: *PBXIP1 modulates embryonic stem cell differentiation by interacting with PBX1*
**作者**: Kawakami Y et al.
**摘要**: 利用重组PBXIP1蛋白进行互作实验，发现其与转录因子PBX1直接结合，调控胚胎干细胞分化相关的基因表达网络，揭示了其在早期发育中的关键作用。
3. **文献名称**: *HPIP facilitates ovarian cancer metastasis via FAK/AKT signaling*
**作者**: Li S et al.
**摘要**: 研究通过体外表达重组HPIP蛋白，证明其激活FAK/AKT通路，增强卵巢癌细胞迁移和侵袭能力，提示靶向HPIP可能抑制转移。
4. **文献名称**: *Structural insights into HPIP functional domains using recombinant protein analysis*
**作者**: Patel R et al.
**摘要**: 通过重组表达并纯化HPIP蛋白片段，结合X射线晶体学鉴定其与微管结合的N端结构域，为解析其在细胞骨架调控中的机制提供结构基础。
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注：以上文献信息为示例，实际引用时需以真实发表文献为准。研究主题覆盖信号通路、蛋白互作、疾病机制及结构分析，体现PBXIP1的多功能性和潜在应用价值。
**PBXIP1 (Pre-B-Cell Leukemia Homeobox Interacting Protein 1)**, also known as HPIP, is a multifunctional protein involved in transcriptional regulation, signal transduction, and cellular processes such as proliferation, differentiation, and migration. It was initially identified as an interaction partner of PBX1. a homeodomain transcription factor critical for embryonic development and organogenesis. PBXIP1 lacks DNA-binding domains but acts as a co-regulator by modulating the activity of transcription factors like PBX1. estrogen receptors, and NF-κB, influencing gene expression programs in development and disease.
Structurally, PBXIP1 contains nuclear localization signals and interaction motifs that facilitate its shuttling between the nucleus and cytoplasm. In the nucleus, it recruits chromatin modifiers to regulate target genes, while cytoplasmic PBXIP1 interacts with cytoskeletal proteins and signaling molecules, such as mTOR and β-catenin, to control cell motility and survival pathways. Dysregulation of PBXIP1 is implicated in cancers, including breast, liver, and colorectal malignancies, where it often promotes tumor growth, metastasis, and chemoresistance via pathways like Wnt/β-catenin and PI3K/AKT. Its roles in stem cell maintenance and immune responses are also emerging areas of study, highlighting its broad physiological and pathological significance.
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