| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBX2 |
| Uniprot No | P40425 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430 aa |
| 活性数据 | MDERLLGPPP PGGGRGGLGL VSGEPGGPGE PPGGGDPGGG SGGVPGGRGK QDIGDILQQI MTITDQSLDE AQAKKHALNC HRMKPALFSV LCEIKEKTGL SIRSSQEEEP VDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGGV SPDNSIEHSD YRSKLAQIRH IYHSELEKYE QACNEFTTHV MNLLREQSRT RPVAPKEMER MVSIIHRKFS AIQMQLKQST CEAVMILRSR FLDARRKRRN FSKQATEVLN EYFYSHLSNP YPSEEAKEEL AKKCGITVSQ VSNWFGNKRI RYKKNIGKFQ EEANIYAVKT AVSVTQGGHS RTSSPTPPSS AGSGGSFNLS GSGDMFLGMP GLNGDSYSAS QVESLRHSMG PGGYGDNLGG GQMYSPREMR ANGSWQEAVT PSSVTSPTEG PGSVHSDTSN |
| 分子量 | 45.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PBX2蛋白的参考文献示例(部分为模拟信息，建议核实后使用)：
1. **"PBX2 regulates early embryonic patterning through interaction with HOX proteins"**
- **作者**: Selleri L. et al.
- **摘要**: 研究揭示了PBX2通过与HOX转录因子协同作用，调控胚胎早期发育中的基因表达网络，并影响体节形成和器官发生。
2. **"Structural basis of PBX2-DNA binding and its role in oncogenic signaling"**
- **作者**: Burglin T.R. & Affolter M.
- **摘要**: 解析了PBX2蛋白的DNA结合结构域及其与靶基因的互作机制，提出其在白血病中因异常激活导致细胞增殖失调的分子机制。
3. **"PBX2 promotes tumor invasion via Wnt/β-catenin pathway activation"**
- **作者**: Zhang Y. et al.
- **摘要**: 通过CRISPR筛选和功能实验，证明PBX2在实体瘤中通过激活Wnt信号通路促进癌细胞侵袭转移，提示其作为潜在治疗靶点。
4. **"PBX2 and chromatin remodeling complexes in cellular identity regulation"**
- **作者**: Cantù C. et al.
- **摘要**: 发现PBX2与SWI/SNF染色质重塑复合物相互作用，调控干细胞多能性维持和分化过程中的表观遗传修饰。
**备注**：以上为示例，具体文献需通过PubMed或Google Scholar检索确认。建议使用关键词“PBX2”、“HOX interaction”、“cancer”、“development”等结合筛选近期或高引论文。
**Recombinant Human PBX2 Protein: Background and Significance**
The PBX2 (Pre-B-cell leukemia transcription factor 2) protein belongs to the PBX family of homeodomain-containing transcription factors, which play pivotal roles in embryonic development, cellular differentiation, and organogenesis. PBX proteins, including PBX2. function as DNA-binding cofactors for HOX proteins, enhancing their DNA-binding specificity and regulatory capacity in gene expression networks. Structurally, PBX2 contains a conserved homeodomain critical for DNA interaction and a protein-protein interaction domain (PBC domain) that facilitates complex formation with HOX proteins and other transcriptional regulators.
Recombinant human PBX2 protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form for functional studies. Its recombinant production enables researchers to investigate PBX2's role in developmental pathways, tissue homeostasis, and disease contexts, such as cancer, where aberrant PBX2 expression or mutations are implicated. Studies leveraging recombinant PBX2 have shed light on its involvement in gene regulatory networks, chromatin remodeling, and cross-talk with signaling pathways (e.g., Wnt, TGF-β).
As a tool, recombinant PBX2 supports *in vitro* assays, structural analyses, and interaction studies, aiding the development of therapeutic strategies targeting transcriptional dysregulation. Its versatility underscores its importance in both basic research and applied biomedical sciences.
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