| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAXIP1 |
| Uniprot No | Q6ZW49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757 aa |
| 活性数据 | MAAGQNLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN |
| 分子量 | 113.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PAXIP1蛋白的3篇代表性文献，摘要内容简洁概括：
1. **"Cryo-EM structure of the human PAXIP1/PTIP-associated chromatin remodeling complex"**
- **作者**: Wang, Y. et al.
- **摘要**: 通过冷冻电镜解析了PAXIP1与MLL3/MLL4组蛋白甲基转移酶复合物的结构，阐明了PAXIP1在染色质重塑中的作用，并发现其通过结构域介导关键蛋白相互作用。
2. **"PTIP regulates the transcriptional activity of p53 by modulating its acetylation"**
- **作者**: Gong, W. et al.
- **摘要**: 研究发现重组PAXIP1蛋白通过招募组蛋白乙酰转移酶p300调控p53的乙酰化水平，从而影响DNA损伤后p53靶基因的转录激活，揭示其在肿瘤抑制中的功能。
3. **"Structural basis for phosphorylation-dependent recruitment of PTIP to DNA damage sites"**
- **作者**: Feng, L. et al.
- **摘要**: 报道了PAXIP1蛋白的磷酸化识别机制，证明重组PAXIP1通过其BRCT结构域结合磷酸化的MDC1.参与DNA损伤修复信号通路的招募过程。
**注**：上述文献信息为示例性概括，实际引用需核实具体文献来源。建议通过PubMed或Web of Science检索关键词“PAXIP1”或“PTIP” + “recombinant”获取最新研究。
PAXIP1 (PAX Interacting Protein 1), also known as PTIP or MGC4. is a nuclear protein that plays critical roles in chromatin modification, DNA repair, and transcriptional regulation. It interacts with Pax transcription factors (e.g., Pax2. Pax6) to regulate gene expression during development, particularly in tissue differentiation and organogenesis. Structurally, PAXIP1 contains six BRCT domains, which mediate protein-protein interactions, and a nuclear localization signal essential for its function in DNA damage response pathways.
A key player in genome stability, PAXIP1 associates with the MLL3/MLL4 histone methyltransferase complexes to facilitate histone H3K4 trimethylation, promoting chromatin accessibility for transcriptional activation or repair machinery. It is indispensable in homologous recombination repair (HRR) of DNA double-strand breaks, collaborating with BRCA1 and 53BP1. Additionally, PAXIP1 is involved in class switch recombination in B-cells, highlighting its significance in adaptive immunity.
Dysregulation of PAXIP1 links to cancer progression, particularly breast cancer and glioblastoma, where its overexpression or mutation may impair DNA repair, driving genomic instability. Murine models show embryonic lethality upon PAXIP1 knockout, underscoring its developmental necessity. Current research focuses on its dual roles in epigenetics and DNA repair, positioning PAXIP1 as a potential therapeutic target for cancers or disorders linked to genome maintenance defects.
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