| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBX3 |
| Uniprot No | P40426 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434 aa |
| 活性数据 | MDDQSRMLQT LAGVNLAGHS VQGGMALPPP PHGHEGADGD GRKQDIGDIL HQIMTITDQS LDEAQAKKHA LNCHRMKPAL FSVLCEIKEK TGLSIRGAQE EDPPDPQLMR LDNMLLAEGV SGPEKGGGSA AAAAAAAASG GSSDNSIEHS DYRAKLTQIR QIYHTELEKY EQACNEFTTH VMNLLREQSR TRPISPKEIE RMVGIIHRKF SSIQMQLKQS TCEAVMILRS RFLDARRKRR NFSKQATEIL NEYFYSHLSN PYPSEEAKEE LAKKCSITVS QVSNWFGNKR IRYKKNIGKF QEEANLYAAK TAVTAAHAVA AAVQNNQTNS PTTPNSGSSG SFNLPNSGDM FMNMQSLNGD SYQGSQVGAN VQSQVDTLRH VINQTGGYSD GLGGNSLYSP HNLNANGGWQ DATTPSSVTS PTEGPGSVHS DTSN |
| 分子量 | 47.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人PBX3蛋白的模拟参考文献示例(仅供格式参考，非真实文献)：
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1. **文献名称**：*Recombinant Human PBX3 Protein Enhances Leukemic Cell Proliferation via HOX Interaction*
**作者**：Zhang L, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化了重组人PBX3蛋白，发现其与HOXA9蛋白在体外特异性结合，并通过激活Wnt/β-catenin通路促进急性髓系白血病细胞增殖，提示PBX3在白血病发生中的关键作用。
2. **文献名称**：*Structural Characterization of Recombinant PBX3 Homeodomain and DNA-Binding Mechanism*
**作者**：Kimura T, et al.
**摘要**：通过X射线晶体学解析了重组人PBX3蛋白同源结构域的立体结构，揭示了其与DNA靶序列结合的分子机制，为靶向PBX3的小分子抑制剂设计提供了结构基础。
3. **文献名称**：*PBX3 Recombinant Protein Promotes EMT in Colorectal Cancer Cells*
**作者**：Wang Y, et al.
**摘要**：利用昆虫细胞表达系统制备高纯度重组PBX3蛋白，证实其通过诱导上皮-间质转化(EMT)增强结直肠癌细胞迁移和侵袭能力，提示PBX3在肿瘤转移中的潜在调控作用。
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**备注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
Recombinant human PBX3 (Pre-B-cell leukemia homeobox 3) protein is a genetically engineered version of the naturally occurring PBX3 transcription factor, a member of the PBX (PREP) family of homeodomain-containing proteins. PBX3 plays critical roles in embryonic development, cell differentiation, and tissue patterning by regulating gene expression through interactions with HOX proteins and other transcription factors. Structurally, PBX3 contains a conserved homeodomain that facilitates DNA binding and a PBC domain for protein-protein interactions, enabling its function in transcriptional complexes.
In research, recombinant PBX3 is widely used to study its biological mechanisms, including its involvement in organogenesis, hematopoiesis, and stem cell maintenance. Aberrant PBX3 expression has been linked to diseases such as leukemia, solid tumors, and neurodevelopmental disorders, where it may promote oncogenesis by enhancing cell proliferation, survival, and metastasis. Recombinant PBX3 protein, typically produced in bacterial or mammalian expression systems, allows for in vitro functional assays, structural studies, and screening of therapeutic agents targeting PBX3-associated pathways. Its applications extend to exploring tissue regeneration strategies and dissecting transcriptional regulatory networks. Optimizing recombinant PBX3 production ensures high purity and activity, essential for both basic research and potential clinical translation.
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