| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBX1 |
| Uniprot No | P40424 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430 aa |
| 活性数据 | MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE EANIYAAKTA VTATNVSAHG SQANSPSTPN SAGSSSSFNM SNSGDLFMSV QSLNGDSYQG AQVGANVQSQ VDTLRHVISQ TGGYSDGLAA SQMYSPQGIS ANGGWQDATT PSSVTSPTEG PGSVHSDTSN |
| 分子量 | 46.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人PBX1蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human PBX1 in E. coli*
**作者**：Smith JL, et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达重组人PBX1蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其与靶DNA结合的活性，为后续结构及功能研究提供基础。
2. **文献名称**：*Structural Analysis of the PBX1 Homeodomain by X-ray Crystallography*
**作者**：Wang Y, et al.
**摘要**：作者利用重组人PBX1蛋白的DNA结合域进行结晶和X射线衍射分析，揭示了其与特定DNA序列相互作用的关键氨基酸残基，阐明了PBX1在转录调控中的分子机制。
3. **文献名称**：*PBX1 Interaction with HOX Proteins in Hematopoietic Development*
**作者**：Garcia R, et al.
**摘要**：通过体外Pull-down和荧光素酶实验，证明重组PBX1蛋白与HOXA9形成复合物，协同调控造血干细胞分化相关基因，提示其在白血病发生中的潜在作用。
4. **文献名称**：*Recombinant PBX1 as a Tool for Investigating Pancreatic Organogenesis*
**作者**：Tanaka H, et al.
**摘要**：利用昆虫细胞表达系统制备重组PBX1蛋白，发现其通过调控Pdx1等基因表达参与胰腺发育，为糖尿病模型研究提供了分子工具。
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**注**：以上文献及作者为虚构示例，实际引用请通过数据库(如PubMed、Web of Science)查询真实发表的研究。
PBX1 (Pre-B-cell leukemia homeobox 1) is a member of the TALE (Three-Amino acid Loop Extension) family of homeodomain-containing transcription factors, which play pivotal roles in embryonic development and tissue differentiation. Initially identified through its involvement in pre–B-cell leukemia via chromosomal translocations, PBX1 is now recognized as a critical regulator of organogenesis, particularly in skeletal, neural, and hematopoietic systems. It functions by forming heterodimeric complexes with HOX proteins, enhancing their DNA-binding specificity and transcriptional activity to control target gene expression during embryogenesis.
Structurally, PBX1 contains a conserved homeodomain for DNA interaction and protein-protein interaction domains, enabling collaboration with HOX proteins and other cofactors. It modulates developmental pathways such as Wnt, FGF, and retinoic acid signaling, influencing cell proliferation, differentiation, and patterning. Mutations or dysregulation of PBX1 are linked to congenital anomalies, including skeletal malformations, neurodevelopmental disorders, and cancers like leukemia and solid tumors. Its role in maintaining stem cell pluripotency and tissue homeostasis further underscores its biological significance.
Research tools like Pbx1-knockout models highlight its necessity in embryonic viability and organ formation, while ongoing studies explore its therapeutic potential in disease contexts. PBX1’s evolutionary conservation across metazoans emphasizes its fundamental role in developmental biology.
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