| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUF1 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645aa |
| 氨基酸序列 | MWTLVGRGWGCARALAPRATGAALLVAPGPRSAPTLGAAPESWATDRLYSSAEFKEKPDMSRFPVENIRNFSIVAHVDHGKSTLADRLLELTGTIDKTKNNKQVLDKLQVERERGITVKAQTASLFYNCEGKQYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSVIPVINKIDLKNADPERVENQIEKVFDIPSDECIKISAKLGTNVESVLQAIIERIPPPKVHRKNPLRALVFDSTFDQYRGVIANVALFDGVVSKGDKIVSAHTQKTYEVNEVGVLNPNEQPTHKLYAGQVGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKS |
| 分子量 | 98 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GUF1蛋白的虚拟参考文献示例，供参考框架使用(具体研究需查询实际数据库)：
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1. **文献名称**：*Structural insights into human GUF1-mediated mitochondrial translation regulation*
**作者**：Chen L. et al.
**摘要**：本研究通过冷冻电镜解析了重组人GUF1蛋白与线粒体核糖体的复合物结构，揭示其通过GTPase活性调控翻译延伸的分子机制，为线粒体疾病相关突变提供结构基础。
2. **文献名称**：*GUF1 knockout impairs cellular stress response via mTOR pathway dysregulation*
**作者**：Park S. et al.
**摘要**：文章利用CRISPR技术构建GUF1缺失细胞模型，发现重组GUF1回补可恢复线粒体应激下mTOR信号传导异常，证实GUF1在能量应激中的分子伴侣功能。
3. **文献名称**：*Recombinant human GUF1 facilitates ribosome quality control in neurodegenerative models*
**作者**：Müller R. et al.
**摘要**：通过体外表达纯化hGUF1蛋白，验证其与错误折叠线粒体蛋白的互作，证明其通过促进核糖体解聚减缓神经退行性疾病模型中的细胞凋亡。
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**注**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台以"human GUF1 protein"或"GUF1 recombinant"为关键词检索获取。GUF1主要研究集中于线粒体翻译调控及神经系统疾病关联。
Recombinant human GUF1 (GTP-binding Universally Conserved in Fungi and Metazoa 1) protein is a conserved GTPase involved in translational quality control, particularly during mitochondrial protein synthesis. GUF1 homologs are found across eukaryotes and prokaryotes, where they play roles in ribosome rescue and stalled peptide chain recycling. In humans, GUF1 localizes to mitochondria and interacts with the mitochondrial ribosome (mitoribosome) to monitor translation fidelity. Mechanistically, GUF1 binds to stalled ribosomes caused by mRNA truncation, structural errors, or stress conditions, facilitating release of tRNA or peptidyl-tRNA through its GTPase activity. This prevents accumulation of defective translation products and maintains cellular proteostasis.
The recombinant form is typically expressed in *E. coli* systems with affinity tags for purification. Structural studies using recombinant GUF1. including X-ray crystallography and cryo-EM, have revealed its GTPase domain architecture and interaction sites with ribosomal components. Current research focuses on its molecular role in mitochondrial translation recovery pathways and implications in mitochondrial disorders linked to protein synthesis defects. Additionally, GUF1's conserved function offers insights into bacterial stress response mechanisms, enabling cross-species comparisons of translational surveillance systems. Its recombinant production supports functional assays and drug discovery targeting translation-related diseases.
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