| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCY1A3 |
| Uniprot No | Q02108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690aa |
| 氨基酸序列 | MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATMPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMAVKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID |
| 分子量 | 103.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GUCY1A3蛋白的参考文献示例(内容为虚构，仅用于演示格式)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Human GUCY1A3 in a Mammalian Cell System"
**作者**: Zhang Y, et al.
**摘要**: 本研究利用HEK293细胞系统成功表达并纯化了重组人GUCY1A3蛋白，证实其与GUCY1B3亚基形成功能性可溶性鸟苷酸环化酶(sGC)，可在体外被一氧化氮激活，为心血管疾病药物的靶点研究提供了工具。
2. **文献名称**: "Role of Recombinant GUCY1A3 in Platelet Aggregation and Vascular Smooth Muscle Relaxation"
**作者**: Müller C, Schlossmann J.
**摘要**: 通过重组人GUCY1A3蛋白的功能实验，发现其参与调节血小板活化和血管平滑肌松弛，缺失该蛋白的小鼠模型表现出血栓形成倾向，提示其在心血管稳态中的关键作用。
3. **文献名称**: "Structural Insights into Recombinant Human GUCY1A3-B3 Complex by Cryo-EM"
**作者**: Li H, Potter LR.
**摘要**: 利用冷冻电镜解析了重组人GUCY1A3与GUCY1B3异源二聚体的三维结构，揭示了其配体结合域和活化机制，为开发靶向sGC的药物治疗高血压和心衰提供了结构基础。
4. **文献名称**: "Recombinant GUCY1A3 Protein Attenuates Pulmonary Hypertension in Rodent Models"
**作者**: Smith R, et al.
**摘要**: 动物实验表明，外源性重组人GUCY1A3蛋白可通过增强sGC活性改善肺动脉高压模型中的血管重塑，突显其潜在治疗价值。
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**提示**：实际文献请通过PubMed、Google Scholar等数据库检索(关键词：recombinant human GUCY1A3. soluble guanylate cyclase, sGC, cardiovascular disease)，并优先选择近5年内的高影响力期刊研究。
Recombinant human GUCY1A3 protein is a key component of the soluble guanylate cyclase (sGC) enzyme complex, which plays a central role in the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) signaling pathway. GUCY1A3. encoded by the *GUCY1A3* gene, forms a heterodimer with the GUCY1B3 subunit to create the functional sGC. This enzyme acts as a receptor for NO, a critical signaling molecule involved in vascular homeostasis. Upon NO binding, sGC catalyzes the conversion of GTP to cGMP, a secondary messenger regulating smooth muscle relaxation, platelet aggregation, and vasodilation.
Dysregulation of sGC activity, linked to GUCY1A3 mutations or altered expression, has been implicated in cardiovascular diseases, including hypertension, pulmonary arterial hypertension, and atherosclerosis. Recombinant GUCY1A3 protein is typically produced using bacterial or mammalian expression systems for biochemical and pharmacological studies. Its applications include elucidating sGC activation mechanisms, screening for therapeutic modulators (e.g., sGC stimulators or activators for cardiovascular drugs), and modeling disease-associated variants. Structural studies of recombinant GUCY1A3 have also advanced the design of targeted therapies aiming to restore NO-sGC-cGMP pathway function. Research continues to explore its role beyond vascular biology, including potential impacts on neuronal signaling and cancer progression.
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