| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCA1B |
| Uniprot No | Q9UMX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-200aa |
| 氨基酸序列 | GQEFSWEEA EAAGEIDVAE LQEWYKKFVM ECPSGTLFMH EFKRFFKVTD DEEASQYVEG MFRAFDKNGD NTIDFLEYVA ALNLVLRGTL EHKLKWTFKI YDKDGNGCID RLELLNIVEG IYQLKKACRR ELQTEQGQLL TPEEVVDRIF LLVDENGDGQ LSLNEFVEGA RRDKWVMKML QMDMNPSSWL AQQRRKSAMF |
| 分子量 | 23.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GUCA1B蛋白的3篇文献参考及摘要概括：
1. **《Molecular cloning and characterization of a retinal-specific guanylyl cyclase-activating protein》**
- **作者**: Dizhoor, A.M., et al.
- **摘要**: 该研究首次克隆了人GUCA1B基因，并证明其编码的GCAP2蛋白通过钙离子依赖性方式激活视网膜鸟苷酸环化酶，参与光信号转导中cGMP水平的调控(1995)。
2. **《A novel GUCA1B mutation in a family with dominant cone-rod dystrophy》**
- **作者**: Sokal, I., et al.
- **摘要**: 报道了GUCA1B基因突变与常染色体显性遗传性锥杆细胞营养不良的关联，发现突变导致重组蛋白功能异常，干扰视网膜光反应通路(2005)。
3. **《Structural insights into calcium-sensitive regulation of guanylyl cyclase-activating proteins》**
- **作者**: Schulze, C., et al.
- **摘要**: 通过X射线晶体学解析重组GUCA1B蛋白的钙离子结合域结构，揭示了其调控鸟苷酸环化酶活性的分子机制，为理解光传导障碍提供结构基础(1998)。
注：以上文献信息为示例性质，实际引用需核对具体文献来源及准确性。如需近期研究，建议在PubMed或Web of Science中以关键词“recombinant GUCA1B”或“GCAP2 expression”检索。
Guanylate cyclase activator 1B (GUCA1B) is a calcium-binding protein encoded by the *GUCA1B* gene in humans, primarily expressed in retinal photoreceptor cells. It regulates the activity of retinal membrane guanylate cyclases (GCs), enzymes critical for synthesizing cyclic guanosine monophosphate (cGMP) in phototransduction. GUCA1B, also known as GCAP2 (Guanylate Cyclase-Activating Protein 2), modulates GC activity in a Ca²⁺-dependent manner: at low Ca²⁺ levels (dark conditions), it activates GCs to replenish cGMP, sustaining photoreceptor sensitivity; at high Ca²⁺ concentrations (light exposure), this activation is inhibited, facilitating signal termination. This dynamic regulation ensures proper visual signal transduction and adaptation to varying light intensities. Mutations in *GUCA1B* are linked to retinal degenerative disorders, such as cone-rod dystrophy, highlighting its essential role in retinal homeostasis. Recombinant human GUCA1B protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, structural analyses, and drug discovery. Its applications include investigating molecular mechanisms of retinal diseases, screening therapeutic compounds, and developing gene therapies targeting GCAP2-associated pathologies. Purification typically involves affinity chromatography, ensuring high purity and bioactivity for experimental or clinical use.
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