| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCY1B2 |
| Uniprot No | O75343 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617aa |
| 氨基酸序列 | MSGYDRMLRT LGGNLMEFIE NLDALHSYLA LSYQEMNAPS FRVERGADGK MFLHYYSDRS GLCHIVPGII EAVAKDFFDI DVIMDILDMN EEVERTGKKE HVVFLIVQKA HRKMRKTKPK RLQDSQGMER DQEALQAAFL KMKEKYLNVS ACPVKKSHWD VVRSIVMFGK GHLMNTFEPI YPERLWIEEK TFCNAFPFHI VFDESLQVKQ ARVNIQKYVP GLQTQNIQLD EYFSIIHPQV TFNIFSIRRF INSQFVLKTR REMMPVAWQS RTTLKLQGQM IWMESMWCMV YLCSPKLRSL QELEELNMHL SDIAPNDTTR DLILLNQQRL AEIELSNQLE RKKEELQVLS KHLAIEKKKT ETLLYAMLPK HVANQLREGK KVAAGEFKSC TILFSDVVTF TNICTACEPI QIVNVLNSMY SKFDRLTSVH AVYKVETIGD AYMVVGGVPV PIGNHAQRVA NFALGMRISA KEVTNPVTGE PIQLRVGIHT GPVLADVVGD KMPRYCLFGD TVNTASRMES HGLPNKVHLS PTAYRALKNQ GFKIIERGEI EVKGKGRMTT YFLIQNLNAT EDEIMGRSKT PVDHKGSTQK ASLPTTKLQG SVQPSCPEHS SLASWLL |
| 分子量 | 70.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GUCY1B2蛋白的模拟参考文献，基于相关领域的研究方向整理：
1. **《Structural and functional analysis of human soluble guanylate cyclase β2 subunit》**
- **作者**: Zhang, Y. et al. (2020)
- **摘要**: 本研究通过哺乳动物表达系统成功重组表达并纯化人GUCY1B2蛋白，解析其与α1亚基的复合物晶体结构，揭示了其与一氧化氮(NO)信号结合的关键结构域，并验证了其在环磷酸鸟苷(cGMP)生成中的催化活性。
2. **《Expression and characterization of recombinant human GUCY1B2 in E. coli for enzymatic activity screening》**
- **作者**: Lee, J.H. & Kim, S. (2018)
- **摘要**: 报道了一种在大肠杆菌中高效表达重组人GUCY1B2蛋白的优化策略，通过亲和层析和体外酶活实验证明该蛋白具备鸟苷酸环化酶活性，并可作为药物靶点筛选工具。
3. **《Role of GUCY1B2 in vascular smooth muscle cell proliferation and hypertension pathogenesis》**
- **作者**: Chen, L. et al. (2019)
- **摘要**: 利用重组GUCY1B2蛋白探索其在血管平滑肌细胞中的功能，发现其通过调节cGMP信号通路抑制细胞增殖，并在高血压动物模型中观察到该亚基的表达下调。
4. **《Development of a high-yield purification protocol for recombinant human GUCY1B2 protein》**
- **作者**: Müller, R. et al. (2021)
- **摘要**: 提出了一种基于HEK293细胞系统的重组人GUCY1B2蛋白高产量纯化方法，通过质谱和功能分析验证了蛋白的完整性和稳定性，为后续药理研究提供可靠原料。
**备注**：以上文献信息为模拟内容，实际文献需通过PubMed、Google Scholar等平台检索确认。
**Recombinant human GUCY1B2 protein** is a engineered form of the soluble guanylate cyclase (sGC) β2 subunit, encoded by the *GUCY1B2* gene. This enzyme plays a critical role in the nitric oxide (NO)-sGC-cyclic guanosine monophosphate (cGMP) signaling pathway, which regulates vascular tone, blood pressure, and cellular homeostasis. GUCY1B2 typically pairs with an α subunit (e.g., GUCY1A1) to form a heterodimeric sGC complex, functioning as a receptor for NO. Binding of NO to sGC activates its cyclase activity, catalyzing the conversion of GTP to cGMP, a secondary messenger mediating smooth muscle relaxation, neurotransmission, and platelet inhibition.
The *GUCY1B2* gene exhibits tissue-specific expression, primarily in the brain, cardiovascular system, and smooth muscle, suggesting roles in neurovascular functions and cardiovascular disease. Dysregulation of sGC activity is linked to hypertension, atherosclerosis, and pulmonary arterial hypertension. Recombinant GUCY1B2 enables studies on isoform-specific sGC functions, as alternative β subunits (e.g., β1 vs. β2) may differ in regulatory mechanisms or tissue distribution. Produced via bacterial or mammalian expression systems, this protein is essential for structural analysis, drug screening (e.g., sGC stimulators/activators), and deciphering pathological mechanisms. Its applications extend to developing therapies targeting cGMP pathway imbalances.
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