| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCY2F |
| Uniprot No | P51841 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 311-402aa |
| 氨基酸序列 | VLTITVESQEKTFYQAFTEAAARGEIPEKLEFDQVSPLFGTIYNSIYFIAQAMNNAMKENGQAGAASLVQHSRNMQFHGFNQLMRTDSNGNGISEYVILDTNLKEWELHS |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GUCY2F蛋白的虚构参考文献示例(供参考)：
1. **《Functional characterization of recombinant human GUCY2F protein in retinal cGMP signaling》**
- **作者：** Zhang, Y. et al. (2018)
- **摘要：** 研究利用杆状病毒-昆虫细胞系统重组表达人GUCY2F蛋白，分析了其体外鸟苷酸环化酶活性，证明其依赖钙离子激活的特性，并揭示了该蛋白在视网膜光信号转导中的潜在作用。
2. **《Structural insights into GUCY2F mutations linked to autosomal dominant retinal dystrophy》**
- **作者：** Branche, T.A. et al. (2020)
- **摘要：** 通过X射线晶体学解析了重组GUCY2F蛋白的胞内催化域结构，发现致病性突变影响蛋白质二聚化及酶活性，为视网膜色素变性(ADRP)的机制提供分子解释。
3. **《Development of a GUCY2F-deficient cell model for studying cone photoreceptor degeneration》**
- **作者：** Li, H. et al. (2021)
- **摘要：** 利用CRISPR-Cas9技术敲除人视网膜色素上皮细胞中的GUCY2F基因，结合重组蛋白回补实验，验证了GUCY2F在维持视锥细胞cGMP稳态中的关键作用。
4. **《High-yield purification of recombinant human GUCY2F for drug screening applications》**
- **作者：** Kumar, S. et al. (2019)
- **摘要：** 报道了一种基于亲和层析和尺寸排阻色谱的重组GUCY2F蛋白高效纯化方法，成功应用于小分子化合物库筛选，发现了潜在的酶活性调控分子。
**注：** 上述文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索关键词“GUCY2F”或“Guanylate cyclase 2F”获取真实文献。
Recombinant human GUCY2F protein is a engineered form of guanylate cyclase 2F, a membrane-bound enzyme belonging to the family of particulate guanylyl cyclases (pGCs). GUCY2F is primarily expressed in retinal photoreceptor cells, where it plays a critical role in phototransduction by catalyzing the conversion of GTP to cyclic GMP (cGMP), a secondary messenger essential for visual signal amplification and recovery. Structurally, it contains an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular catalytic domain. Unlike other retinal guanylyl cyclases (e.g., GUCY2D), GUCY2F is uniquely regulated by calcium-sensitive proteins and may contribute to maintaining cGMP homeostasis under varying light conditions.
The recombinant protein is produced using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional activity. Its study has implications for understanding retinal degenerative diseases, such as retinitis pigmentosa, and colorectal cancer, where GUCY2F overexpression has been observed. Additionally, recombinant GUCY2F serves as a tool for investigating ligand-receptor interactions, as some pGCs respond to peptide hormones like guanylin. Research efforts focus on its potential as a therapeutic target or diagnostic biomarker, leveraging its role in cellular signaling pathways. However, its precise physiological ligands and regulatory mechanisms outside the retina remain partially characterized, driving ongoing biochemical and structural studies.
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