| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTSE1 |
| Uniprot No | Q9NYZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-720aa |
| 氨基酸序列 | MDDPKKEDILLLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNVPAAGSHLGQGKRAIPVPNKLGLKKTLLKAPGSTSNLARKSSSGPVWSGASSACTSPAVGKAKSSEFASIPANSSRPLSNISKSGRMGPAMLRPALPAGPVGASSWQAKRVDVSELAAEQLTAPPSASPTQPQTPEGGGQWLNSSCAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSARRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF |
| 分子量 | 105.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTSE1蛋白的3篇示例参考文献(模拟文献，仅供参考实际研究需核实)：
1. **文献名称**：GTSE1 regulates p53 stability and chemoresistance in cancer cells
**作者**：Smith A et al.
**摘要**：研究揭示了GTSE1通过结合并促进p53蛋白的泛素化降解，抑制DNA损伤诱导的细胞凋亡，从而增强肿瘤细胞对化疗药物的耐药性。
2. **文献名称**：GTSE1 promotes cell migration via EMT signaling in breast cancer
**作者**：Chen L et al.
**摘要**：该文献发现重组人GTSE1蛋白过表达可通过激活上皮间质转化(EMT)通路，增强乳腺癌细胞的迁移和侵袭能力，并与患者预后不良相关。
3. **文献名称**：Structural analysis of GTSE1-microtubule interaction
**作者**：Yamamoto K et al.
**摘要**：研究利用重组GTSE1蛋白进行体外结合实验，证实其通过N端结构域直接结合微管，调控有丝分裂期纺锤体稳定性，影响细胞周期进程。
注：以上内容为学术示例，实际文献需通过PubMed、Web of Science等平台检索。建议结合具体研究方向(如癌症、细胞周期等)补充关键词筛选。
**Background of Recombinant Human GTSE1 Protein**
GTSE1 (G2 and Three-Phase Stage Expressed Protein 1) is a microtubule-associated protein primarily involved in regulating cell cycle progression, particularly during the G2/M phase and mitotic exit. It interacts with p53. a critical tumor suppressor, by promoting its cytoplasmic sequestration and degradation, thereby modulating DNA damage response and cellular stress pathways. Overexpression of GTSE1 is observed in various cancers, correlating with poor prognosis, tumor progression, and resistance to chemotherapy, likely due to its anti-apoptotic effects and role in sustaining genomic instability.
Recombinant human GTSE1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) using DNA cloning techniques to ensure high purity and biological activity. This engineered protein serves as a vital tool for studying GTSE1's molecular mechanisms, including its interplay with p53. regulation of mitotic checkpoints, and contribution to cancer phenotypes. Additionally, it aids in screening potential inhibitors targeting GTSE1 for anticancer therapy. Research leveraging recombinant GTSE1 continues to explore its dual role as both a oncogenic driver and a therapeutic vulnerability in malignancies.
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