| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCA1A |
| Uniprot No | P43080 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGYIDFMEYVAALSLVLKGKVEQKLRWYFKLYDVDGNGCIDRDELLTIIQAIRAINPCSDTTMTAEEFTDTVFSKIDVNGDGELSLEEFIEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGADEAAEAAG |
| 分子量 | 47.85 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GUCA1A蛋白的参考文献概览：
1. **文献名称**: *Structural and functional analysis of recombinant human GUCA1A reveals mechanisms of Ca²⁺-dependent regulation in retinal phototransduction*
**作者**: Müller, S. et al.
**摘要**: 该研究通过X射线晶体学和功能实验解析了重组人GUCA1A的钙离子依赖性构象变化，揭示了其如何通过结合Ca²⁺调控视网膜鸟苷酸环化酶的活性，从而影响光信号转导。
2. **文献名称**: *Expression and mutagenesis of recombinant GUCA1A in HEK293 cells: Implications for inherited retinal dystrophies*
**作者**: Peshenko, I.V. et al.
**摘要**: 作者利用HEK293细胞表达了重组GUCA1A蛋白，并分析了多个致病突变(如Y99C、I107T)对其结构和功能的破坏，阐明了突变如何导致异常cGMP水平及视网膜变性。
3. **文献名称**: *Calcium-sensitive inhibition of retinal guanylyl cyclase by recombinant human GUCA1A variants*
**作者**: Dizhoor, A.M. et al.
**摘要**: 该文通过重组蛋白实验证明GUCA1A在低钙条件下激活视网膜鸟苷酸环化酶，而在高钙环境下抑制其活性，揭示了其在光适应中的动态调节机制。
4. **文献名称**: *Optimized prokaryotic expression and purification of functional human GUCA1A for biophysical studies*
**作者**: Dell’Orco, D. et al.
**摘要**: 研究提出了一种基于大肠杆菌的高效重组GUCA1A表达和纯化方案，验证了其与靶蛋白的结合能力，为大规模生化和结构研究提供了可靠方法。
**注**：以上文献信息为示例性质，实际引用时请根据具体文章内容核对作者、标题及发表年份。
Recombinant human GUCA1A protein is a engineered form of the guanylyl cyclase-activating protein 1 (GCAP1), encoded by the GUCA1A gene. GCAP1 belongs to a family of calcium-sensitive proteins that regulate retinal membrane guanylyl cyclases (RetGCs) in photoreceptor cells, playing a critical role in phototransduction recovery and light adaptation. Under low calcium conditions (dark-adapted state), GCAP1 activates RetGCs to synthesize cyclic GMP (cGMP), maintaining cGMP-gated ion channel opening for photoreceptor depolarization. When calcium levels rise during light exposure, GCAP1 dissociates from RetGCs, terminating cGMP production and enabling hyperpolarization.
The recombinant protein (rGUCA1A) is typically expressed in bacterial (e.g., *E. coli*) or mammalian systems with tags for purification, retaining native post-translational modifications when produced in eukaryotic cells. Its production enables biochemical studies of calcium-dependent RetGC regulation and disease mechanisms. Mutations in GUCA1A are associated with autosomal dominant cone-rod dystrophy and retinal degeneration, making rGUCA1A valuable for structural analysis of pathogenic variants and therapeutic development. Recent research focuses on its conformational dynamics, metal ion binding properties (Ca²⁺/Mg²⁺), and interactions with RetGC isoforms. Standard applications include calcium sensitivity assays, crystallographic studies, and drug screening platforms for inherited retinal diseases.
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