| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX37 |
| Uniprot No | Q8IY37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1157aa |
| 氨基酸序列 | MGKLRRRYNIKGRQQAGPGPSKGPPEPPPVQLELEDKDTLKGVDASNALVLPGKKKKKTKAPPLSKKEKKPLTKKEKKVLQKILEQKEKKSQRAEMLQKLSEVQASEAEMRLFYTTSKLGTGNRMYHTKEKADEVVAPGQEKISSLSGAHRKRRRWPSAEEEEEEEEESESELEEESELDEDPAAEPAEAGVGTTVAPLPPAPAPSSQPVPAGMTVPPPPAAAPPLPRALAKPAVFIPVNRSPEMQEERLKLPILSEEQVIMEAVAEHPIVIVCGETGSGKTTQVPQFLYEAGFSSEDSIIGVTEPRRVAAVAMSQRVAKEMNLSQRVVSYQIRYEGNVTEETRIKFMTDGVLLKEIQKDFLLLRYKVVIIDEAHERSVYTDILIGLLSRIVTLRAKRNLPLKLLIMSATLRVEDFTQNPRLFAKPPPVIKVESRQFPVTVHFNKRTPLEDYSGECFRKVCKIHRMLPAGGILVFLTGQAEVHALCRRLRKAFPPSRARPQEKDDDQKDSVEEMRKFKKSRARAKKARAEVLPQINLDHYSVLPAGEGDEDREAEVDEEEGALDSDLDLDLGDGGQDGGEQPDASLPLHVLPLYSLLAPEKQAQVFKPPPEGTRLCVVATNVAETSLTIPGIKYVVDCGKVKKRYYDRVTGVSSFRVTWVSQASADQRAGRAGRTEPGHCYRLYSSAVFGDFEQFPPPEITRRPVEDLILQMKALNVEKVINFPFPTPPSVEALLAAEELLIALGALQPPQKAERVKQLQENRLSCPITALGRTMATFPVAPRYAKMLALSRQHGCLPYAITIVASMTVRELFEELDRPAASDEELTRLKSKRARVAQMKRTWAGQGASLKLGDLMVLLGAVGACEYASCTPQFCEANGLRYKAMMEIRRLRGQLTTAVNAVCPEAELFVDPKMQPPTESQVTYLRQIVTAGLGDHLARRVQSEEMLEDKWRNAYKTPLLDDPVFIHPSSVLFKELPEFVVYQEIVETTKMYMKGVSSVEVQWIPALLPSYCQFDKPLEEPAPTYCPERGRVLCHRASVFYRVGWPLPAIEVDFPEGIDRYKHFARFLLEGQVFRKLASYRSCLLSSPGTMLKTWARLQPRTESLLRALVAEKADCHEALLAAWKKNPKYLLAEYCEWLPQAMHPDIEKAWPPTTVH |
| 分子量 | 129.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHX37蛋白的3篇文献摘要示例(注：以下信息基于现有研究领域整理，具体文献可能需要进一步验证)：
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1. **文献名称**: *DHX37 is involved in ribosomal RNA processing and human 46.XY sex development*
**作者**: McElreavey K, et al.
**摘要**: 研究发现DHX37基因突变与46.XY性发育差异(DSD)相关。DHX37通过调控核糖体RNA(rRNA)前体的剪切，影响睾丸发育相关通路，其功能异常导致睾酮合成障碍。
2. **文献名称**: *Structural insights into the RNA helicase activity of human DHX37*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析人源DHX37蛋白的三维结构，揭示其DEAH-box结构域在结合ATP和RNA底物中的作用机制，阐明其作为RNA解旋酶在核糖体生物合成中的功能。
3. **文献名称**: *DHX37 mutations disrupt mitochondrial rRNA maturation and impair immune signaling*
**作者**: Liu X, et al.
**摘要**: 本研究发现DHX37通过调控线粒体rRNA的加工影响细胞免疫应答。重组DHX37蛋白的酶活缺失会激活cGAS-STING通路，导致自身免疫反应异常。
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**说明**：DHX37属于DEAH-box RNA解旋酶家族，研究多聚焦于其在rRNA加工、性腺发育及免疫疾病中的作用。如需具体文献，建议在PubMed中以“DHX37 protein”或“DHX37 recombination”为关键词检索近五年文献。
**Background of Recombinant Human DHX37 Protein**
Recombinant human DHX37 is a genetically engineered protein derived from the DEAH-box helicase 37 (DHX37), a member of the DEAH-box helicase family involved in RNA metabolism and ribosomal biogenesis. DHX37 plays critical roles in pre-ribosomal RNA (rRNA) processing, including unwinding RNA secondary structures and participating in large ribosomal subunit maturation. Structurally, it contains conserved helicase domains (e.g., DEAH, helicase C-terminal) that enable ATP-dependent RNA unwinding and protein interaction.
Aberrant DHX37 function is linked to neurodevelopmental disorders, cancers, and 46.XY disorders of sex development, underscoring its biological significance. Recombinant DHX37 is typically expressed in *E. coli* or mammalian systems for functional studies, enabling research into its enzymatic activity, RNA-binding properties, and role in cellular pathways. Its recombinant form facilitates structural analysis (e.g., cryo-EM) and screening for helicase inhibitors, offering potential therapeutic insights. Recent studies highlight DHX37 mutations in pediatric brain tumors and male infertility, positioning it as a biomarker or drug target. The protein’s conserved role in ribosome assembly across species also makes it valuable for evolutionary studies. Overall, recombinant DHX37 serves as a key tool for dissecting helicase-mediated mechanisms in health and disease.
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