| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | MYF6; BHLHC4; MRF4; Myogenic factor 6; Myf-6; Class C basic helix-loop-helix protein 4; bHLHc4; Muscle-specific regulatory factor 4 |
| Entrez GeneID | 4618; |
| WB Predicted band size | 26kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human Myf-6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于Myf-6抗体的3篇参考文献示例(内容基于假设文献整理，仅供参考)：
1. **文献名称**: "Monoclonal antibodies specific for the myogenic regulatory protein Myf-6"
**作者**: Hinterberger, T.J., et al.
**摘要**: 该研究报道了针对Myf-6蛋白的单克隆抗体制备，验证了其在免疫印迹(Western blot)和免疫组化中的特异性，并证明其在体外肌肉分化模型中检测Myf-6表达的应用。
2. **文献名称**: "Differential expression of Myf-6 and other myogenic regulators in mouse skeletal muscle"
**作者**: Yee, S.P., Rigby, P.W.
**摘要**: 通过Myf-6抗体分析小鼠骨骼肌发育不同阶段MRF家族(MyoD、Myogenin、Myf-5、Myf-6)的表达模式，发现Myf-6在成熟肌纤维中持续高表达，提示其可能参与维持肌肉稳态。
3. **文献名称**: "Myf-6 antibody reveals regenerative myofiber nuclei in muscular dystrophy models"
**作者**: Smith, J.L., Miller, J.B.
**摘要**: 利用Myf-6特异性抗体研究肌营养不良症模型，发现再生肌纤维的细胞核中Myf-6蛋白显著上调，提示其在肌肉修复中的潜在作用。
(注：若需真实文献，建议在PubMed或Google Scholar中检索关键词"Myf-6 antibody"或"MRF4 antibody"筛选近期研究。)
The Myf-6 antibody is a tool used to detect Myf-6 (myogenic factor 6), a member of the MyoD family of transcription factors critical for skeletal muscle development and differentiation. Also known as MRF4 (muscle regulatory factor 4) or herculin, Myf-6 is encoded by the *MYF6* gene and plays a role in regulating myogenesis, particularly during late-stage muscle cell maturation and maintenance. Alongside other myogenic regulatory factors (MRFs) like MyoD, Myf-5. and myogenin, Myf-6 contributes to the activation of muscle-specific genes and the transition of myoblasts into terminally differentiated myotubes.
The Myf-6 antibody is commonly utilized in research to study muscle development, regeneration, and diseases such as muscular dystrophies or sarcopenia. It enables the identification and localization of Myf-6 protein in tissues or cells via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Studies using this antibody have elucidated Myf-6's dual role in both promoting differentiation and maintaining the quiescent state of satellite cells, depending on cellular context.
Produced in various host species (e.g., rabbit, mouse), Myf-6 antibodies are validated for specificity and sensitivity in model organisms, including mice and humans. Their applications extend to developmental biology, cancer research (e.g., rhabdomyosarcoma), and regenerative medicine, offering insights into transcriptional regulation of muscle-specific pathways. Reliable detection of Myf-6 aids in understanding its interplay with other MRFs and its potential as a therapeutic target for muscle-related disorders.
×
