| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | STK24; MST3; STK3; Serine/threonine-protein kinase 24; Mammalian STE20-like protein kinase 3; MST-3; STE20-like kinase MST3 |
| Entrez GeneID | 8428; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human MST-3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MST-3抗体的3篇参考文献示例(注：MST-3相关研究较少，部分内容为假设性概括，建议通过学术数据库核实)：
1. **"Characterization of a Novel Monoclonal Antibody Against MST-3 Kinase and Its Application in Tumor Cell Apoptosis Studies"**
- **作者**: Smith A, et al.
- **摘要**: 本研究开发了一种针对MST-3激酶的新型单克隆抗体，通过免疫印迹和免疫荧光验证其特异性。结果显示，该抗体可有效检测细胞中MST-3的表达，并揭示其在DNA损伤诱导的肿瘤细胞凋亡中的关键作用。
2. **"MST-3 Antibody-Based Detection of Hippo Pathway Activity in Developmental Biology"**
- **作者**: Zhang L, et al.
- **摘要**: 利用MST-3特异性抗体研究Hippo信号通路在胚胎发育中的功能，发现MST-3通过磷酸化下游效应蛋白YAP调控组织器官大小，抗体在斑马鱼模型中的应用证实了其跨物种适用性。
3. **"Comparative Analysis of MST-3 Isoforms Using Isoform-Specific Antibodies in Neurodegenerative Models"**
- **作者**: Tanaka K, et al.
- **摘要**: 研究设计两种针对MST-3不同剪接变体的抗体，发现MST-3α在阿尔茨海默病模型中异常激活，与tau蛋白过度磷酸化相关，提示其作为治疗靶点的潜力。
**注意事项**：
- MST-3(Mammalian Sterile 20-like kinase 3)可能与STK24等激酶存在命名混淆，建议结合具体研究背景筛选文献。
- 上述内容为简化示例，实际文献需通过PubMed、Web of Science等平台以关键词“MST-3 antibody”“STK24 antibody”或“Hippo pathway MST-3”检索。
The MST-3 antibody targets the mammalian sterile 20-like kinase 3 (MST3. also known as STK24), a serine/threonine kinase belonging to the GCK-III subfamily within the STE20 kinase family. MST3 plays a critical role in regulating cellular processes such as apoptosis, cell polarity, and stress responses. It is activated by autophosphorylation and participates in signaling pathways linked to energy stress, redox balance, and Hippo pathway modulation. MST3 interacts with proteins like STRAD and CCM3. influencing cytoskeletal dynamics and endothelial cell functions. Dysregulation of MST3 is implicated in pathologies including cerebral cavernous malformations (CCMs) and cancer, where it may act as a tumor suppressor or promoter depending on context.
The MST-3 antibody is widely used in research to detect endogenous MST3 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Its development has facilitated studies exploring MST3’s tissue distribution, activation states, and interaction networks. Validated antibodies exhibit specificity for distinct phosphorylation sites or conformational states, enabling insights into kinase activity. Recent studies highlight MST3’s role in metabolic disorders and neurodegenerative diseases, expanding its therapeutic relevance. Researchers rely on MST-3 antibodies to dissect its dual roles in cell survival/death and its crosstalk with pathways like AMPK and mTOR, underscoring its potential as a biomarker or drug target.
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