| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MRPS12; RPMS12; RPSM12; 28S ribosomal protein S12; mitochondrial; MRP-S12; S12mt; MT-RPS12 |
| Entrez GeneID | 6183; |
| WB Predicted band size | 20kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human MRP-S12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MRP-S12抗体的假设性参考文献示例(基于常见研究方向，仅供参考)：
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1. **文献名称**：*MRP-S12 regulates mitochondrial translation and apoptosis in mammalian cells*
**作者**：Smith J, et al.
**摘要**：本研究利用特异性MRP-S12抗体，通过Western blot和免疫荧光技术，揭示MRP-S12在线粒体翻译中的关键作用。实验表明，MRP-S12缺失导致线粒体膜电位下降并触发细胞凋亡。
2. **文献名称**：*Aberrant expression of MRP-S12 in colorectal cancer and its clinical implications*
**作者**：Zhang L, et al.
**摘要**：通过免疫组化分析MRP-S12在结直肠癌组织中的表达，发现其高表达与肿瘤转移和患者生存率降低相关，提示其可能作为潜在预后标志物。
3. **文献名称**：*Development and validation of a polyclonal antibody against human MRP-S12 for functional studies*
**作者**：Lee S, et al.
**摘要**：报道了一种兔源多克隆MRP-S12抗体的制备与验证，该抗体适用于免疫沉淀和细胞定位，为研究线粒体核糖体蛋白功能提供了工具。
4. **文献名称**：*MRP-S12 mutations impair mitochondrial protein synthesis and cause neurodegenerative disorders*
**作者**：Patel R, et al.
**摘要**：结合CRISPR基因编辑与MRP-S12抗体检测，发现该蛋白的突变导致线粒体翻译缺陷，并与早发性神经退行性疾病相关。
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**注**：以上文献为示例性内容，实际引用需查询真实数据库(如PubMed)。若需具体文献，建议使用关键词“MRP-S12 antibody”或“Mitochondrial Ribosomal Protein S12”进一步检索。
The MRP-S12 antibody is a research tool designed to target the mitochondrial ribosomal protein S12 (MRP-S12), a component of the mitochondrial ribosome essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translation machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which generate ATP. MRP-S12 is encoded by nuclear DNA, translated in the cytoplasm, and imported into mitochondria, where it integrates into the 28S small ribosomal subunit. This protein plays a critical role in maintaining mitochondrial ribosome stability and ensuring accurate translation of mitochondrial-encoded mRNAs.
Antibodies against MRP-S12 are widely used to study mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s), and metabolic syndromes. Researchers employ these antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess MRP-S12 expression levels, subcellular localization, and interactions with other mitochondrial components. Reduced MRP-S12 levels are often associated with impaired mitochondrial respiration, increased oxidative stress, and apoptosis, making it a biomarker for mitochondrial health.
Commercial MRP-S12 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins. Validation includes testing on knockout cell lines or tissues to confirm specificity. Its applications extend to drug discovery, particularly in therapies targeting mitochondrial diseases or cancer metabolism.
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