关键信息
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基因名
NRAS
- 应用
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别名
NRAS;HRAS1;GTPase NRas
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P01111
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表达区间
1-186aa
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氨基酸序列
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPC
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分子量
56.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NRAS is a member of the RAS oncogene family and plays a crucial role in cellular signaling pathways that regulate cell growth, differentiation, and survival. Mutations in the NRAS gene are frequently associated with various types of cancers, particularly melanoma, and hematological malignancies, making it a significant target for cancer research. The study of NRAS recombinant proteins is essential for understanding the molecular mechanisms underlying these cancers, as well as for the development of targeted therapies. By producing and analyzing NRAS recombinant proteins, researchers can investigate the protein's structure, function, and interactions with other cellular molecules, which may reveal potential therapeutic targets or biomarkers for treatment resistance. Furthermore, NRAS is known to interact with various downstream effectors, including the MAPK and PI3K pathways, contributing to its oncogenic potential. Enhanced insights into NRAS protein dynamics could lead to the identification of novel inhibitors that specifically disrupt its activity, offering new avenues for cancer treatment. In summary, the investigation of NRAS recombinant proteins not only furthers our understanding of cancer biology but also paves the way for innovative therapeutic strategies against NRAS-driven tumors.
