| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 14 beta; 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; kinase p70S6K-beta; KS6B2 |
| Entrez GeneID | 6199; |
| WB Predicted band size | 42kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human p70 S6 Kinase β around the phosphorylation site of serine 423 (P-V-S(p)-P-L). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于p70S6 Kinase β (Ab-423)抗体的参考文献(示例为虚构，实际需根据真实文献补充)：
---
1. **文献名称**: *"mTORC1-dependent phosphorylation of p70S6Kβ regulates cell growth in glioblastoma"*
**作者**: Smith J, et al.
**摘要**: 研究利用p70S6Kβ (Ab-423)抗体检测mTORC1信号激活状态，发现其在胶质母细胞瘤细胞增殖中的关键作用，证实Ab-423抗体特异性识别Ser423磷酸化位点，为靶向治疗提供依据。
2. **文献名称**: *"Role of p70S6Kβ in skeletal muscle hypertrophy via Ab-423 phosphorylation analysis"*
**作者**: Lee H, et al.
**摘要**: 通过Western blot(使用Ab-423抗体)分析肌肉细胞中p70S6Kβ的活性，揭示其在机械负荷诱导的蛋白质合成中的调控机制，验证抗体在组织样本中的高特异性。
3. **文献名称**: *"Validation of p70S6Kβ antibody (Ab-423) in human cancer cell lines"*
**作者**: Patel R, et al.
**摘要**: 系统评估Ab-423抗体在多种癌细胞系中的性能，确认其适用于免疫沉淀及免疫荧光实验，并证明p70S6Kβ磷酸化水平与化疗耐药性相关。
---
(注：以上文献为示例，实际引用需检索PubMed/Google Scholar等数据库，建议用关键词“p70S6Kβ antibody Ab-423”或“S6K1 phosphorylation Ser423”查找真实研究。)
The p70 S6 Kinase β (Ab-423) antibody is a specific immunological tool designed to detect the phosphorylated form of p70 S6 Kinase β (p70S6Kβ), a serine/threonine kinase central to the mTOR (mechanistic target of rapamycin) signaling pathway. This antibody specifically recognizes the phosphorylated residue at Thr423 (or a homologous site depending on isoform), a critical post-translational modification associated with kinase activation. p70S6Kβ, encoded by the *RPS6KB2* gene, is one of two isoforms (α and β) of p70 S6 kinase. Both isoforms regulate cell growth, proliferation, and survival by phosphorylating downstream targets such as the S6 ribosomal protein, which enhances protein synthesis. The β isoform shares functional overlap with p70S6Kα but exhibits distinct tissue expression patterns and regulatory roles.
The Ab-423 antibody is widely used in research to study mTOR pathway activity, particularly in contexts like cancer, metabolic disorders, and autophagy. It enables detection of activated p70S6Kβ via techniques such as Western blotting, immunofluorescence, or immunoprecipitation, providing insights into signaling dynamics under experimental conditions (e.g., nutrient availability, drug treatment). Its specificity for the phosphorylated form makes it valuable for assessing pathway activation status, validating mTOR inhibitor efficacy, or exploring dysregulation in disease models. Proper controls, including phosphorylation site blocking peptides or kinase inhibition assays, are recommended to confirm signal specificity.
×
