| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; Large tumor suppressor 1; WARTS; |
| Entrez GeneID | 9113; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human LATS1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LATS1/2抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*"LATS1/2 suppress NF2 mutant tumorigenesis by controlling Hippo pathway activation"*
**作者**：Li et al.
**摘要内容**：该研究利用LATS1/2特异性抗体，通过Western blot和免疫组化分析，揭示了LATS1/2在神经纤维瘤病(NF2)突变肿瘤中通过调控Hippo通路活性抑制肿瘤发生的机制，证明其缺失导致YAP/TAZ异常激活。
2. **文献名称**：*"Regulation of YAP by phosphorylation-dependent LATS1/2 binding"*
**作者**：Hao et al.
**摘要内容**：作者使用磷酸化特异性LATS1/2抗体，验证了LATS1/2激酶在细胞周期进程中通过磷酸化YAP并促进其胞质滞留的功能，强调了抗体在检测磷酸化依赖性相互作用中的关键作用。
3. **文献名称**：*"LATS2 antagonizes mitosis exit via interaction with PP1 to regulate Cdk1 phosphorylation"*
**作者**：Yang et al.
**摘要内容**：该研究通过LATS2抗体进行免疫共沉淀(Co-IP)和染色质定位分析，发现LATS2通过与磷酸酶PP1结合调控Cdk1磷酸化，延缓有丝分裂退出，为癌症治疗提供了潜在靶点。
这些文献均通过LATS1/2抗体在分子机制研究中发挥重要作用，涵盖肿瘤抑制、信号通路调控及细胞周期分析。建议通过PubMed或期刊官网输入标题或作者名获取全文。
LATS1 (large tumor suppressor kinase 1) and LATS2 are serine/threonine kinases that play critical roles in the Hippo signaling pathway, a conserved regulatory network controlling cell proliferation, apoptosis, and organ size. Discovered as mammalian orthologs of the Drosophila tumor suppressor gene *warts*, LATS1/2 function as tumor suppressors by phosphorylating downstream effectors like YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif), thereby inhibiting their nuclear translocation and oncogenic transcriptional activity. Structurally, LATS1/2 contain a central kinase domain, regulatory regions, and conserved motifs for interaction with upstream regulators like MST1/2 kinases.
Antibodies targeting LATS1/2 are essential tools for studying Hippo pathway dynamics. They enable detection of endogenous protein expression, post-translational modifications (e.g., phosphorylation at specific residues like Thr1079/1041 in LATS1/2), and subcellular localization in various experimental models. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate their roles in development, tissue homeostasis, and diseases like cancer. However, challenges remain due to sequence homology between LATS1 and LATS2 (~50% identity), requiring careful validation of antibody specificity. Cross-reactivity or isoform-selective detection must be confirmed using knockout controls. Additionally, phosphorylation-dependent antibodies are critical for assessing kinase activation status, as Hippo signaling is tightly regulated by phosphorylation cascades. Such reagents have advanced research into therapeutic targeting of YAP/TAZ in cancers and regenerative medicine.
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